| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Outline of Final Research Achievements |
IL-33 is expressed in the nucleus of epithelial and endothelial cells. In this study, it was investigated that the effect of IL-33 knockdown (KD) with siRNA in normal human epidermal keratinocytes (NHEKs). IL-33 KD attenuated BrdU uptake.The cell cycle analysis found that the number of cells in G2/M phase was increased in IL-33 KD cells.Immunofluorescence study revealed that there were many binucleated cells in IL33 KD cell culture, which were scarcely found in control cells. Western blot analysis revealed that IL-33 KD cells showed attenuated myosin light chain phosphorylation, which was reported to associate with contractile ring formation. Reduced expression of Ect2 was observed, which would have caused reduced activation of RhoA. These results suggest that nuclear IL-33 is positively involved in cell proliferation through promoting contractile ring formation by positively regulating myosin light chain phosphorylation, expression of Ect2, and activation of RhoA in NHEKs.
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