| Project/Area Number |
25461712
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Dermatology
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| Research Institution | Juntendo University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
OKA Akira 東海大学, 医科学研究所, 講師 (80384866)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 円形脱毛症 / マイクロサテライト / GWAS / ゲノム解析 / ゲノム編集 / 疾患遺伝子 / 遺伝子 / ノックアウトマウス / ノックインマウス / alopecia areata / gene |
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| Outline of Final Research Achievements |
During the fiscal year of 2013-2015, we have done genome-wide association study of alopecia areata (AA) patients by utilizing microsatellite markers. In addition, we have also examined by using SNP markers and next-generation sequencing, and have obtained below-mentioned results.
1) We have identified strongest association of AA at HLA-C locus within HLA lesion (OR: 3.41). 2) 28 haplotypes were detected, and 1 of them was found to contain responsible gene for AA. 3) 5 risk and 3 non-risk haplotypes were examined by next generation sequencer, and an exonic non-synonymous single nucleotide variant, which was common to all risk haplotype, was only detected. We are now trying to generate gene modified mouse model, and examine its phenotype.
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