Is keratoacanthoma a benign neoplasm?: Retrieval of heterogenity using proteomics.

• Project/Area Number: 25461716
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Dermatology
• Research Institution: Nippon Medical School
• Principal Investigator: Ansai Shin-ichi 日本医科大学, 医学部, 教授 (10159519)
• Co-Investigator(Kenkyū-buntansha): NAITO Zenya 日本医科大学, 大学院医学研究科, 教授 (20237184) ; KUDO Mitsuhiro 日本医科大学, 医学部, 講師 (20256978)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: ケラトアカントーマ / 有棘細胞癌 / クレーター状疣贅 / IMP3 / MAPK4 / クレーター状上皮性腫瘍 / crateriform verruca / keratoacanthoma like SCC / malignant transformation / keratoacanthoma / IGF2BP / プロテオミクス

Outline of Final Research Achievements

We classified the tumors with crateriform silhouette like keratoacanthoma (KA) using large amounts of cases. Of these, there were about 20％ cases that showed distinctive histopathological feature different from KA. Whereas 56.3% of the cases were KA, about 10% of those were KA with malignant transformation and about 10% of other crateriform malignant neoplasms were also contained.
Insulinlike growth factor 2 mRNAbinding protein-3;(IMP3) was positive in about 50% of the cases with SCC, though none of KA was positive. IMP is thought to be new target for treatment and useful marker for differentiation between SCC and KA.

Research Products

• [Journal Article] A clinicopathologic study of crateriform verruca: crateriform epithelial lesions histopathologically distinct from keratoacanthoma (2016)
  • Author(s): Ogita A, AnsaiS, Misago N, Anan T, Fukumoto T, Saeki H
  • Journal Title: The Journal of Dermatology Volume: 43 Issue: 10 Pages: 0-0
  • DOI: 10.1111/1346-8138.13331
  • Peer Reviewed
• [Journal Article] Histopathological diagnosis of epithelial crateriform tumors: keratoacanthoma and other epithelial crateriform tumors (2016)
  • Author(s): Ogita A, AnsaiS, Misago N, Anan T, Fukumoto T, Saeki H
  • Journal Title: The Journal of Dermatology Volume: 43 Issue: 11 Pages: 0-0
  • DOI: 10.1111/1346-8138.13390
  • Peer Reviewed
• [Journal Article] Insulin-like growth factor 2 mRNA-binding protein-3 as a marker for distinguishing between cutaneous squamous cell carcinoma and keratoacanthoma. (2016)
  • Author(s): Kanzaki A, Kudo M, Ansai S, Peng WX, Ishino K, Yamamoto T, Wada R, Fujii T, Teduka K, Kawahara K, Kawamoto Y, Kitamura T, Kawana S, Saeki H, Naito Z.
  • Journal Title: Int J Oncol Volume: 48 Issue: 3 Pages: 1007-15
  • DOI: 10.3892/ijo.2016.3323
  • Peer Reviewed / Open Access
• [Journal Article] 単発性ケラトアカントーマ：Solitary Keratoacanthomaと臨床診断された症例の病理組織診断について (2013)
  • Author(s): 安齋眞一、福本隆也、阿南隆、木村鉄宣、川名誠司
  • Journal Title: 日本皮膚科学会雑誌 Volume: 123 Pages: 1775-1784
  • Peer Reviewed
• [Presentation] FFPE切片を用いたケラトアカントーマのプロテオーム解析 (2014)
  • Author(s): 神崎亜希子、工藤光洋、安齋眞一、山本哲志、藤井雄文、手塚潔、彭為霞、和田龍一、佐伯秀久、内藤善哉
  • Organizer: 第73回日本癌学会学術総会
  • Place of Presentation: 横浜市
  • Year and Date: 2014-09-25 – 2014-09-27
• [Presentation] FFPE組織を用いたプロテオーム解析によるkeratoacanthoma-like SCCとSCCの比較検討 (2014)
  • Author(s): 紺野亜希子、工藤光洋、安齋眞一、山本哲志、藤井雄文、手塚潔、彭為霞、川名誠司、内藤善哉
  • Organizer: 第103回日本病理学会総会
  • Place of Presentation: 広島市
  • Year and Date: 2014-04-24 – 2014-04-26
• [Presentation] FFPE 切片を用いたケラトアカントーマのプロテオーム解析 (2013)
  • Author(s): 紺野 亜希子、工藤 光洋、安齋 眞一、山本 哲志、藤井 雄文、手塚 潔、彭 為霞、川名 誠司、内藤 善哉
  • Organizer: 第72回 日本癌学会学術総会
  • Place of Presentation: 横浜、パシフィコ横浜
• [Presentation] ケラントアカントーマのホルマリン固定パラフィン包埋組織によるプロテオーム解析 (2013)
  • Author(s): 紺野 亜希子、工藤 光洋、山本 哲志、藤井 雄文、手塚 潔、彭 為霞、安斎 眞一、川名 誠司、内藤 善哉
  • Organizer: 第102回 日本病理学会総会
  • Place of Presentation: 札幌、ロイトン札幌
• [Book] 皮膚科臨床アセット17 皮膚悪性腫瘍 のうち 有棘細胞癌、日光角化症、Bowen病、ケラトアカントーマの診断と治療 (2014)
  • Author(s): 安齋眞一
  • Publisher: 中山書店