Glucose metabolism in malignant lymphoma; its application for treatment

• Project/Area Number: 25461802
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Radiation science
• Research Institution: Gunma University
• Principal Investigator: Tsukamoto Norifumi 群馬大学, 医学部附属病院, 准教授 (10292583)
• Co-Investigator(Kenkyū-buntansha): YOKOHAMA Akihiko 群馬大学, 医学部附属病院, 准教授 (40323365) ; MITSI TAKEKI 群馬大学, 医学部附属病院, 助教 (80420181)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2013: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: 悪性リンパ腫 / グルコース代謝 / グルコーストランスポーター / 免疫組織化学染色 / リアルタイムPCR / hexokinase / FDG-PET / ヘキソキナーゼ / FDG / 免疫組織化学 / DLBCL / グルコース / 解糖系 / リンパ腫

Outline of Final Research Achievements

Expression of GLUT1 to GLUT12 on lymphoma cells using real-time PCR was analyzed and reveal that GLUT5 expression was enhanced in some lymphoma cell lines. Based on this datum, shRNA vector was constructed and its effect on cell growth and glucose metabolism is investigating.
Then, immunohistological staining of GLUT1, GLUT3, GLUT5, and hexokinase II were performed. In diffuse large cell lymphoma patients, either GLUT1 or GLUT3 was positive, while GLUT5 and hexokinase II were partially positive. The relation to clinical data, such as SUVmax level on FDG-PET scan and prognosis, is now analyzing.

Research Products

• [Journal Article] SF3B1 and IGHV gene mutation status predict poor prognosis in Japanese CLL patients. (2016)
  • Author(s): Mitsui T, Koiso H, Nakahashi H, Saitoh A, Shimizu H, Ishizaki T, Ogawa Y, Takizawa M, Yokohama A, Saitoh T, Jinbo T, Ogura H, Handa H, Sawamura M, Sakura T, Karasawa M0, Murakami H, Nojima Y, Tsukamoto N.
  • Journal Title: Int J Hematol. Volume: 103 Issue: 2 Pages: 219-226
  • DOI: 10.1007/s12185-015-1912-z
  • Peer Reviewed
• [Journal Article] Ki-67 is a strong predictor of central nervous system relapse in patients with mantle cell lymphoma (MCL). (2015)
  • Author(s): Chihara D, Asano N, Ohmachi K, Nishikori M, Okamoto M, Sawa M, Sakai R, Okoshi Y, Tsukamoto N, Yakushijin Y, Nakamura S, Kinoshita T, Ogura M, Suzuki R.
  • Journal Title: Ann Oncol. Volume: 未定 Issue: 5 Pages: 966-973
  • DOI: 10.1093/annonc/mdv074
  • Peer Reviewed / Open Access
• [Journal Article] Gene polymorphisms of mannose-binding lectin confer susceptibility to Pneumocystis pneumonia in HIV-infected patients. (2015)
  • Author(s): Yanagisawa K, Ogawa Y, Uchiumi H, Gohda F, Mawatari M, Ishizaki T, Mitsui T, Yokohama A, Handa H, Tsukamoto N, Nojima Y.
  • Journal Title: J Infect Chemother. Volume: 21 Issue: 11 Pages: 769-775
  • DOI: 10.1016/j.jiac.2015.07.006
  • Peer Reviewed
• [Journal Article] Biological significance of fluorine-18-α-methyltyrosine (FAMT) uptake on PET in patients with oesophageal cancer. (2014)
  • Author(s): Suzuki S, Kaira K, Ohshima Y, Ishioka NS, Sohda M, Yokobori T, Miyazaki T, Oriuchi N, Tominaga H, Kanai Y, Tsukamoto N, Asao T, Tsushima Y, Higuchi T, Oyama T, Kuwano H.
  • Journal Title: Br J Cancer Volume: Epub ahead of print Issue: 8 Pages: 1985-1991
  • DOI: 10.1038/bjc.2014.142
  • Peer Reviewed / Open Access
• [Journal Article] CD98 as a novel prognostic indicator for patients with stage III/IV hypopharyngeal squamous cell carcinoma. (2014)
  • Author(s): Toyoda M, Kaira K, Shino M, Sakakura K, Takahashi K, Takayasu Y, Tominaga H, Oriuchi N, Nikkuni O, Suzuki M, Iijima M, Tsukamoto N, Nagamori S, Kanai Y, Oyama T, Chikamatsu K.
  • Journal Title: Head Neck. Volume: in press Issue: 11 Pages: 1-6
  • DOI: 10.1002/hed.23797
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] A new staging system to predict prognosis of patients with multiple myeloma in an era of novel therapeutic agents. (2014)
  • Author(s): Iriuchishima H, Saitoh T, Handa H, Isoda A, Matsumoto M, Sawamura M, Iwasaki A, Ushie C, Hattori H, Sasaki Y, Mitsui T, Yokohama A, Tsukamoto N, Murakami H, Nojima Y.
  • Journal Title: Eur J Haematol. Volume: 94 Issue: 2 Pages: 145-151
  • DOI: 10.1111/ejh.12407
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Regulatory T cells percentage in peripheral blood before and after eradication of Helicobacter pylori. (2014)
  • Author(s): Satoh Y, Ogawara H, Kawamura O, Shimoyama Y, Kusano M, Yokohama A, Saitoh T, Handa H, Tsukamoto N, Murakami H.
  • Journal Title: Health Volume: 6 Pages: 236-242
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Biological significance of fluorine-18-α-methyltyrosine (FAMT) uptake on PET in patients with oesophageal (2014)
  • Author(s): Suzuki S, Kaira K, Ohshima Y, Ishioka NS, Sohda M, Yokobori T, Miyazaki T, Oriuchi , Tominaga H, Kanai Y, Tsukamoto N, Asao T, Tsushima Y, Higuchi T, Oyama , Kuwano H
  • Journal Title: Br J Cancer Volume: 110 Pages: 1985-1991
  • Peer Reviewed
• [Journal Article] Relapsed primary intraocular lymphoma treated with intravitreal methotrexate and high-dose chemotherapy followed by autologous stem cell rescue. (2014)
  • Author(s): Kamio T, Yokohama A, Hayashi T, Toyama K, Koya H, Hoshino T, Saitoh T, Hanyuda N, Mukai R, Handa H, Murakami H, Tsukamoto N, Nojima Y.
  • Journal Title: Rinsho Ketsueki Volume: 55 Pages: 244-248
  • NAID: 130004920658
  • Peer Reviewed
• [Journal Article] Prognostic importance of the soluble form of IL-2 receptorα (sIL-2Rα) and its relationship with surface expression of IL-2Rα (CD25) of lymphoma cells in diffuse large B-cell lymphoma treated with CHOP-like regimen with or without rituximab: a retrospective analysis of 338 cases. (2013)
  • Author(s): Hashimoto Y, Yokohama A, Saitoh A, Nakahashi H, Toyama K, Mitsui T, Koiso H, Saitoh T, Handa H, Uchiumi H, Jinbo T, Murayama K, Matsumoto M, Sawamura M, Karasawa M, Murakami H, Hirato J, Nojima Y, Kojima M, Tsukamoto N
  • Journal Title: J Clin Exp Hematop Volume: 53 Pages: 197-205
  • NAID: 130003383994
  • Peer Reviewed
• [Journal Article] Characterization of CD56+ dendritic-like cells: a normal counterpart of blastic plasmacytoid dendritic cell neoplasm? (2013)
  • Author(s): Osaki Y, Yokohama A, Saito A, Tahara K, Yanagisawa K, Ogawa Y, Ishizaki T, Mitsui T, Koiso H, Takizawa M, Uchiumi H, Saitoh T, Handa H, Murakami H, Tsukamoto N, Nojima Y.
  • Journal Title: PLoS One. Volume: 8 Issue: 11 Pages: e81722-e81722
  • DOI: 10.1371/journal.pone.0081722
  • Peer Reviewed
• [Journal Article] Development of IgG4-related disease 10 years after chemotherapy for diffuse large B cell lymphoma and longstanding bronchial asthma. (2013)
  • Author(s): Mitsui T, Yokohama A, Koiso H, Ishizaki T, Uchiumi H, Saitoh T, Handa H, Hirato J, Karasawa M, Murakami H, Kojima M, Nojima Y, Tsukamoto N.
  • Journal Title: Int J Hematol. Volume: 98 Issue: 1 Pages: 122-128
  • DOI: 10.1007/s12185-013-1359-z
  • Peer Reviewed
• [Journal Article] Recurrent Gemella haemolysans meningitis in a patient with osteomyelitis of the clivus. (2013)
  • Author(s): Hayashi T, Uchiumi H, Yanagisawa K, Ogawa Y, Handa H, Tsukamoto N, Nojima Y.
  • Journal Title: Intern Med. Volume: 52 Pages: 2145-2147
  • NAID: 130003365816
  • Peer Reviewed
• [Presentation] Soluble IL-2 receptor α is a more sensitive diagnostic biomarker than β2 microglobulin and predicts survival in follicular lymphoma: Retrospective analysis of 305 cases. (2014)
  • Author(s): Yokohama A, Hashimoto Y, Hiroaki S, Saito A, Miyazawa Y, Iriujijima H, Toyama K, Ishizaki T, Mitsui T, Koiso H, Takizawa M, Saito T, Handa H, Murayama K, Matsumoto M, Sawamura M, Murakami H, Nojima Y, Hirato J, Kojima M, Tsukamoto N.
  • Organizer: 米国血液学会
  • Place of Presentation: サンフランシスコ
  • Year and Date: 2014-12-06 – 2014-12-09
• [Presentation] PAX5 rearrangement in a primary intraocular lymphoma. (2014)
  • Author(s): Koiso H, Yokohama A, Ishizaki T, Mitsui T, Takizawa M, Saitoh T, Handa H, Morimoto M, Murakami H, Hirato J, Kojima M, Nojima Y, Tsukamoto N.
  • Organizer: 日本血液学会総会
  • Place of Presentation: 大阪
  • Year and Date: 2014-10-31 – 2014-11-02
• [Presentation] 非典型的な初発症状を呈した全身性Marginal zone lymphomaの3例. (2014)
  • Author(s): 滝沢牧子、横濱章彦、斉藤明生、三井健揮、星野匠臣、小磯博美、斉藤貴之、半田寛、村上博和、塚本憲史、村山佳予子、平戸純子、小島勝.
  • Organizer: リンパ網内系学会総会
  • Place of Presentation: 山形
  • Year and Date: 2014-06-19 – 2014-06-21
• [Presentation] Peripheral blood T cell subsets during the chemotherapy for malignant lymphoma. (2013)
  • Author(s): Omiya C, Koiso H, Kamiya A, Watanabe S, Saitoh t, Tsukamoto N, Yokohama A, Mitsui T, Handa H, Murakami H.
  • Organizer: 第75回日本血液学会総会
  • Place of Presentation: 札幌
• [Presentation] Bese excision repair gene polymorphisms affect the clinical feature of Myelodyspoastic syndromes. (2013)
  • Author(s): Iwasaki A, Saitoh T, Ushie C, Narjmeg B, Nitta Y, Masuda Y, Kaneko A, Suda I, Hoshino T, Takada S, Sakura T, Yokohama A, Tsukamoto N, Handa H, Murakami H.
  • Organizer: 第75回日本血液学会総会
  • Place of Presentation: 札幌
• [Presentation] Mutation of the SF3B1 predict a poor prognosis in Japanese CLL patients. (2013)
  • Author(s): Mitsui T, Koiso H, Nakahashi H, Saito A, Ishizaki T, Takizawa M, Yokohama A, Saito T, Jinbo T, Ogura H, Handa H, Sawamura M, Sakura T, Karasawa M, Murakami H, Nojima Y, Tsukamoto N.
  • Organizer: 第75回日本血液学会総会2013
  • Place of Presentation: 札幌