Formulation of PET radiopharmaceuticals using solid phase extraction method

• Project/Area Number: 25461845
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Radiation science
• Research Institution: Iwate Medical University
• Principal Investigator: TERASAKI Kazunori 岩手医科大学, 医学部, 講師 (60285632)
• Co-Investigator(Kenkyū-buntansha): SHOZUSHIMA Masanori 岩手医科大学, 歯科放射線学分野, 教授 (00118259)
• Co-Investigator(Renkei-kenkyūsha): IWATA Ren 東北大学, サイクロトロンラジオアイソトープセンター, 教授 (60143038)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2015: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: PET / 放射性薬剤 / 固相抽出 / 核薬学 / PET製剤

Outline of Final Research Achievements

Solid phase extraction (SPE) was used for the formulation of several PET radiopharmaceuticals as an alternative to the rotary evaporator methods. The SPE formulation process were optimized for dilution of the purified HPLC fraction with water, the selection of SPE cartridge, washing the cartridge with water, elution of the radiopharmaceutical with a small volume of ethanol and dilution with isotonic saline. The percentage of ethanol in the final solution was adjusted 2.5% (v/v) of volume. In all cases, a high radiochemical purity (>95%), residual organic solvent levels in the injectable solutions were below the recommended limits. The stability and the solubility of in the final solutions containing 2.5% of ethanol were maintained in practical time limit. The formulation by the SPE method enabled the efficiency and labor-saving in the manufacturing operation.

Research Products

• [Journal Article] High-uptake areas on positron emission tomography with the hypoxic radiotracer 18F-FRP170 in glioblastomas include regions retaining proliferative activity under hypoxia. (2015)
  • Author(s): Beppu T, Sasaki T, Terasaki K, Saura H, Mtsuura H, Ogasawara K, Sasaki M, Ehara S, Iwata R, Takai Y
  • Journal Title: Ann Nucl Med Volume: 29 Issue: 4 Pages: 336-341
  • DOI: 10.1007/s12149-015-0951-0
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Hypoxic viable tissue in human chronic cerebral ischemia because of unilateral major cerebral artery steno-occlusive disease. (2015)
  • Author(s): Saura H, Ogasawara K, Beppu T, Yoshida K, Kobayashi M, Yoshida K, Terasaki K, Takai Y, Ogawa A.
  • Journal Title: Stroke Volume: 46 Issue: 5 Pages: 1250-1256
  • DOI: 10.1161/strokeaha.114.008238
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Post-carotid endarterectomy changes in cerebral glucose metabolism on 18F-fluorodeoxyglucose positron emission tomography associated with postoperative improvement or impairment in cognitive function. (2015)
  • Author(s): Yoshida K, Ogasawara K, Saura H, Saito H, Kobayashi M, Yoshida K, Terasaki K, Fujiwara S, Ogawa A.
  • Journal Title: J Neurosurg Volume: 31 Issue: 6 Pages: 1-9
  • DOI: 10.3171/2014.12.jns142339
  • Peer Reviewed
• [Journal Article] 18F-AV45（Florbetapir F18）を用いた認知症における脳アミロイドイメージング (2015)
  • Author(s): 柴田俊秀、米澤久司、高橋純子、工藤 雅子、小原智子、鈴木真紗子、石塚直樹、藤澤 豊、佐々木敏秋、寺崎一典、世良耕一郎、寺山靖夫
  • Journal Title: 脳循環代謝 Volume: 26 Pages: 11-18
  • NAID: 130005093691
  • Peer Reviewed
• [Journal Article] Biological characteristics of a cerebellar mass regrowing after removal in a patient with lhermitte-duclos disease: emission tomography studies. (2014)
  • Author(s): Saito H, Ogasawara K, Beppu T, Matsuura H, Terasaki K.
  • Journal Title: Case Rep Neurol Volume: 6 Issue: 1 Pages: 96-100
  • DOI: 10.1159/000362118
  • Peer Reviewed
• [Journal Article] Combination of blood flow asymmetry in the cerebral and cerebellar hemispheres on brain perfusion SPECT predicts 5-year outcome in patients with symptomatic unilateral major cerebral artery occlusion. (2014)
  • Author(s): Nomura J, Ogasawara K, Saito H, Terasaki K, Matsumoto Y, Takahashi Y, Ogasawara Y, Saura H, Yoshida K, Sato Y, Kubo Y, Ogawa A
  • Journal Title: Neurol Res. Volume: 36(3) Issue: 3 Pages: 262-9
  • DOI: 10.1179/1743132813y.0000000300
  • Peer Reviewed
• [Journal Article] Combination of blood flow asymmetry in the cerebral and cerebellar hemispheres on brain perfusion SPECT predicts 5-year outcome in patients with symptomatic unilateral major cerebral artery occlusion (2014)
  • Author(s): Nomura J
  • Journal Title: Neurol Res Volume: 36 Pages: 262-269
  • Peer Reviewed
• [Journal Article] Standardized uptake value in high uptake area on positron emission tomography with 18F-FRP170 as a hypoxic cell tracer correlates with intratumoral oxygen pressure in glioblastoma (2014)
  • Author(s): Beppu T, Terasaki K, Sasaki T, Fujiwara S, Matsuura H, Ogasawara K, Sera K, Yamada N, Uesugi N, Sugai T, Kudo K, Sasaki M, Ehara S, Iwata R, Takai Y
  • Journal Title: Mol Imaging Biol Volume: 16 Issue: 1 Pages: 127-135
  • DOI: 10.1007/s11307-013-0670-7
  • Peer Reviewed
• [Journal Article] Detection of misery perfusion in the cerebral hemisphere with chronic unilateral major cerebral artery steno-occlusive disease using crossed cerebellar hypoperfusion: comparison of brain SPECT and PET imaging. (2013)
  • Author(s): Matsumoto Y, Ogasawara K, Saito H, Terasaki K, Takahashi Y, Ogasawara Y, Kobayashi M, Yoshida K, Beppu T, Kubo Y, Fujiwara S, Tsushima E, Ogawa A
  • Journal Title: Eur J Nucl Med Mol Imaging. Volume: 40(10) Issue: 10 Pages: 1573-81
  • DOI: 10.1007/s00259-013-2463-2
  • Peer Reviewed
• [Journal Article] Combined measurement of cerebral and cerebellar blood flow on preoperative brain perfusion SPECT imaging predicts development of new cerebral ischemic events after endarterectomy for symptomatic unilateral cervical carotid stenosis. (2013)
  • Author(s): Oikawa K, Ogasawara K, Saito H, Yoshida K, Saura H, Sato Y, Terasaki K, Wada T, Kubo Y.
  • Journal Title: Clin Nucl Med Volume: 38 Issue: 12 Pages: 957-961
  • DOI: 10.1097/rlu.0000000000000279
  • Peer Reviewed
• [Presentation] 11C-標識プローブの実用的なマイクロリアクター合成法の開発 (2015)
  • Author(s): 石川洋一、岩田 錬、寺崎一典
  • Organizer: 第55回日本核医学会学術総会
  • Place of Presentation: 東京
  • Year and Date: 2015-11-05
• [Presentation] 3DDRAMA法を使用した分解能補正効果の検討 (2015)
  • Author(s): 佐々木敏秋、寺崎一典、世良耕一郎
  • Organizer: 第55回日本核医学会学術総会
  • Place of Presentation: 東京
  • Year and Date: 2015-11-05
• [Presentation] Post-carotid endarterectomy changes of cerebral glucose metabolism on 18F-fluoro-deoxyglucose positron emission tomography associated with postoperative improvement and impairment in cognitive function (2015)
  • Author(s): Yoshida K, Ogasawara K, Saura H, Saito H, Kobayashi M, Yoshida K, Terasaki K, Fujiwara S, Ogawa A
  • Organizer: 7th Japanese-Korean Joint Stroke Conference
  • Place of Presentation: Busan, Korea
  • Year and Date: 2015-10-22
  • Int'l Joint Research
• [Presentation] Hypoxic viable tissue in human chronic cerebral ischemia because of unilateral major cerebral artery steno-occlusive disease (2015)
  • Author(s): Saura H, Ogasawara K, Beppu T, Yoshida K, Kobayashi M, Yoshida K, Terasaki K, Takai Y, obayashi M, Ogawa A
  • Organizer: 7th Japanese-Korean Joint Stroke Conference
  • Place of Presentation: Busan, Korea
  • Year and Date: 2015-10-22
  • Int'l Joint Research
• [Presentation] Frequency of pseudo-responce on MRI at the rarly stage during BEVACIZUMAB treatment for recurrent glioblastoma: comparisons with biological respones on L-methyl-11C-methionine PET (2015)
  • Author(s): T. Beppu, T. Sasaki, K. Terasaki, H. Matsuura, K. Ogasawara
  • Organizer: 38th European Society of Neuroradiology Annual Meeting
  • Place of Presentation: Naples, Italy
  • Year and Date: 2015-09-17
  • Int'l Joint Research
• [Presentation] [11C]メチオニンの効率的、信頼性の高い製造法の開発：オンカラム標識法と固相抽出による製剤化 (2014)
  • Author(s): 寺崎一典、石川洋一、小豆島正典、世良耕一郎、岩田 錬．
  • Organizer: 第54回核医学会学術総会
  • Place of Presentation: 大阪
  • Year and Date: 2014-11-06 – 2014-11-08
• [Presentation] [11C]メチオニンのオンカラム法の最適化と固相抽出法による製剤化の検討 (2013)
  • Author(s): 寺崎一典
  • Organizer: 第53回日本核医学会学術総会
  • Place of Presentation: 福岡市
• [Remarks] NMCC共同利用研究成果報文集
  • URL: http://www.jrias.or.jp/association/cat9/sub-nishina/