| Project/Area Number |
25461851
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Radiation science
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| Research Institution | Kanazawa Medical University |
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Principal Investigator |
DOAI Mariko 金沢医科大学, 医学部, 助教 (40515673)
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| Co-Investigator(Kenkyū-buntansha) |
WATANABE Naoto 金沢医科大学, 医学部, 教授 (40210926)
IWABUCHI Kuniyoshi 金沢医科大学, 医学部, 教授 (10232696)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | ストロンチウム / 放射線組織障害 / γ-H2AX / リンパ球 / アポトーシス |
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| Outline of Final Research Achievements |
The purpose of this study was to investgate the degree of radiotoxicity to lymphocytes following strontium-89 (Sr-89) therapy for bone metastases using γ-H2AX foci immunodetection. In addition, the degree of radiotoxicity to apoptosis of lymphocytes was also examined. This study was compared the γ-H2AX focus incidence in in vivo and in vitro irradiated lymphocytes to estimate the effective absorbed radiation dose to lymphocytes in vivo. As a result, the radiation-induced DNA damage to the lymphocytes exposed to Sr-89 could be detectable using γH2AX foci immunodetection.
The apoptototic cells may also facilitate estimation of the radiotoxicity with this therapy. The γ-H2AX foci immunodetection and detection of apoptotic cells may facilitate estimation of the radiation doses absorbed and the degree of radiotoxicity to lymphocytes with this therapy
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