| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2015: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Outline of Final Research Achievements |
To evaluate the efficacy of intraarterial infusion of CO2-saturated solution, 50 ml of solution (saline solution and CO2-saturated solution for the control and CO2 groups, respectively) was administered close to the feeding artery of the VX2 tumors, that were implanted to fourteen Japanese white rabbits 3 weeks before. Rabbits were divided into control and CO2 groups. All rabbits were killed for tumor harvest on day 3 after the procedure. Tumor volume was evaluated with contrast-enhanced computed tomography (CECT). Tumor apoptotic changes were examined by DNA fragmentation assay and immunoblot analysis. Tumor growth ratio was significantly decreased in the CO2 group compared with the control group. Apoptotic activity in the CO2 group was higher than in the control group. Intraarterial infusion of CO2-saturated solution inhibits rabbit VX2 thigh tumor growth by activation of apoptotic cell death through cleaved caspase-3 upregulation.
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