| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Outline of Final Research Achievements |
The purpose of this study was to investigate the feasibility of imaging brain redox status using a 11C-labeled dihydroquinoline derivative ([11C]DHQ1) for positron emission tomography. The lipophilic [11C]DHQ1 was rapidly oxidized to its hydrophilic form in mouse brain homogenate. The redox modulators diphenyleneiodonium and apocynin significantly decreased the oxidation velocity of [11C]DHQ1, and apocynin caused concentration-dependent inhibition of the oxidation velocity. Furthermore, [11C]DHQ1 diffused into the brain after administration and underwent oxidation into the hydrophilic metabolite, which then slowly decreased. By contrast, apocynin treatment caused a rapid decrease of radioactivity in the brain and inhibited the in vivo oxidation of [11C]DHQ1 to the hydrophilic metabolite. Thus, [11C]DHQ1 is a potential tracer for imaging of redox status in the brain.
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