Induction of functional exsomes selectively derived from mesenchymal stem cells
| Project/Area Number |
25461896
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Radiation science
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| Research Institution | National Institute of Radiological Sciences |
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Principal Investigator |
Tajima Katsushi 国立研究開発法人放射線医学総合研究所, 緊急被ばく医療研究センター, プログラムリーダー (80292423)
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| Co-Investigator(Kenkyū-buntansha) |
YASUDA Takeshi 国立研究開発法人放射線医学総合研究所, 緊急被ばく医療研究センター, 主任研究員 (60332269)
GOTOH Takaya 大東文化大学, スポーツ健康科学部, 教授 (80284355)
羽澤 勝治 金沢大学, 学内共同利用施設等, 助教 (40622460)
早乙女 愛 国立研究開発法人放射線医学総合研究所, その他部局等, 研究員 (80462688)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Keywords | 間葉系幹細胞 / 再生医療 / エキソソーム / 放射線 / 放射線生物学 / Exosome / mMesenchymal stem cell / radiation / regeneration / エクソゾーム / テトラスパン / インテグリン / 再生 |
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| Outline of Final Research Achievements |
We investigated effects and mechanisms of mesenchymal stem cells (MSCs) against irradiated cells. Exosomes derived from MSCs inhibited cell-death of irradiated cells. Radiation increased cellular uptake of exosomes dependent on its doses, and induced CD29/CD81 complex formation on cellular surfaces. Exosomes were entered into cells through selectively binding its complex. The cellular uptake of exosomes were not dependent on phagocytosis, endocytosis, and pinocytosis. A factor “X” and exosomes from MSCs protected intestinal epithelial cell injury using radiation- exposure model animals. We demonstrated the mechanisms of MSCs on irradiated cells.
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Report
(4 results)
Research Products
(13 results)
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[Journal Article] A prospective analysis of clinical efficacy and safety in chronic myeloid leukemia-chronic phase patients with imatinib resistance or intolerance as evaluated using European Leukemia Net 2013 criteria2015
Author(s)
Murai K, Akagi T, Shimosegawa K, Sugawara T, Ishizawa K, Ito S, Motegi M, Yokoyama H, Noji H, Tajima K, Chou T, Ogawa K, Harigae H, Kubo K, Ooba k, Sakamoto J, Ishida Y
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Journal Title
Eur J Haematol
Volume: 10
Pages: 12536-12536
Related Report
Peer Reviewed / Open Access
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[Journal Article] A prospective analysis of clinical efficacy and safety in chronic myeloid leukemia-chronic phase patients with imatinib resistance or intolerance as evaluated using European LeukemiaNet 2013 criteria2015
Author(s)
2.Murai K, Akagi T, Shimosegawa K, Sugawara T, Ishizawa K, Ito S, Murai K, Motegi M, Yokoyama H, Noji H, Tajima K, Kimura J, Chou T, Ogawa K, Harigae H, Kubo K, Oba K, Sakamoto J, Ishida Y
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Journal Title
Eur J Haematol
Volume: Feb 19
Issue: 6
Pages: 12536-12536
DOI
Related Report
Peer Reviewed / Open Access
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