Basic study of the peroxide production in mouse's tissue by X-ray irradiation, and protective effects of the anti-oxidizing agent(extracts from basidiomycetes) on radiation damages.

• Project/Area Number: 25461906
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Radiation science
• Research Institution: Chiba University
• Principal Investigator: UNO TAKASHI 千葉大学, 医学(系)研究科(研究院), 教授 (30302540)
• Co-Investigator(Kenkyū-buntansha): HORIKOSHI TAKURO 千葉大学, 医学部附属病院, 助教 (50456068) ; HARADA RINTARO 千葉大学, 医学部附属病院, 助教 (20568662) ; SAITO MASAYOSHI 千葉大学, 大学院医学研究院, 特任研究員 (80118885) ; INOUE KOUHEI 千葉大学, 医学部附属病院, 助教 (40312053) ; ISOBE KOUICHI 東邦大学, 医学部, 准教授 (80334184)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 放射線治療生物学 / 過酸化物 / 放射線防護 / DNA過酸化物 / 8-OHdG / 8-BrdG / 担子菌抽出物 / 放射線障害 / 過酸化物生成物 / マウス / 延命効果 / 全身照射

Outline of Final Research Achievements

This present study was planned to investigate on peroxide productions (8-OHdG and 8-BrdG) after the X-ray exposure to mice, and protection effects from radiation damages by antioxidant prescription (extracts from basidiomycota : ”extracts”).
(1) The peroxide of DNA (8-OHdG) was detected in 4.5Gy irradiated mice plasma for 5 to 7 days after irradiation. The positive spots (8-OHdG) of thymus and spleen preparations in mice were detected in 5 days after irradiation. The positive spots of an oxidative stress marker (8-BrdG) in thymus, spleen were detected in 1 day and/or 3days after irradiation. (2) The survival rate of the male ICR mice exposed to 8.0Gy whole-body X-ray irradiation, all administered groups of “extracts” were increase the survival rate. The radiation death was caused by the radiation intestinal damages (sepsis by mouse’s enteric bacteria). The sperm count and lymphocyte activity in irradiated mice were suggested to elevated by “extracts” administration.

Research Products

• [Journal Article] Early-onset dropped head syndrome after radiotherapy for head and neck cancer: dose constraints for neck extensor muscles. (2016)
  • Author(s): Inaba K, Nakamura S, Okamoto H, Kashihara T, Kobayashi K, Harada K, Kitaguchi M, Sekii S, Takahashi K, Murakami N, Ito Y, Igaki H, Uno T, Itami J.
  • Journal Title: J Radiat Res. Volume: 57 Pages: 169-173
  • Peer Reviewed
• [Journal Article] New approach to relieving pain and distress during high-dose-rate intracavitary irradiation for cervical cancer. (2015)
  • Author(s): Watanabe Nemoto M, Nozaki-Taguchi N, Togasaki G, Kanazawa A, Kurokawa M, Harada R, Kobayashi H, Isono S, Uno T.
  • Journal Title: Brachytherapy. Volume: 14 Pages: 642-647
  • Peer Reviewed
• [Journal Article] Daily low-dose Cisplatin-based concurrent chemoradiotherapy for the treatment of cervical cancer in patiants 70 years or older. (2015)
  • Author(s): Hanawa S, Mitsuhashi A, Usui H, Yamamoto N, Watanabe-Nemoto M, Nishikimi K, Uehara T, Tate S, Uno T, Shozu M.
  • Journal Title: Int J Gynecol Cancer. Volume: 25 Pages: 891-896
  • Peer Reviewed
• [Journal Article] Initial experience of radiotherapy plus cetuximab for Japanese head and neck cancer patients. (2015)
  • Author(s): Kurokawa M, Watanabe Nemoto M, Harada R, Kobayashi H, Horikoshi T, Kanazawa A, Togasaki G, Abe Y, Chazono H, Hanazawa T, Okamoto Y, Uno T.
  • Journal Title: J Radiat Res. Volume: 56 Pages: 849-855
  • Peer Reviewed
• [Journal Article] Comprehensive Registry of Esophageal Cancer in Japan (2014)
  • Author(s): Tachimori Y, Ozawa S, Fujishiro M, Matsubara H, Numasaki H, Oyama T, Shinoda M, Toh Y, Udagawa H, Uno T.
  • Journal Title: Esophagus Volume: 11 Pages: 21-47
  • Peer Reviewed
• [Journal Article] Localized ocular adnexal mucosa-associated lymphoid tissue lymphoma treated with radiation therapy: a long-term outcome in 86 patients with 104 treated eyes (2014)
  • Author(s): Harada K, Murakami N, Kitaguchi M, Sekii S, Takahashi K, Yoshio K, Inaba K, Morota M, Ito Y, Sumi M, Suzuki S, Tobinai K, Uno T, Itami J.
  • Journal Title: International Journal of Radiattion Oncology biology･ｐhysics Volume: 88 Pages: 650-4
  • Peer Reviewed
• [Journal Article] Japanese structure survey of high-precision radiotherapy in 2012 based on institutional questionnaire about the patterns of care. (2014)
  • Author(s): Tomita N, Kodaira T, Teshima T, Ogawa K, Kumazaki Y, Yamauchi C, Toita T, Uno T, Sumi M, Onishi H, Kenjo M, Nakamura K.
  • Journal Title: Japanese Journal of Clinical Oncology Volume: 44 Pages: 579-86
  • Peer Reviewed
• [Journal Article] Delayed renal dysfunction after total body irradiation in pediatric malignancies. (2014)
  • Author(s): Watanabe Nemoto M, Isobe K, Togasaki G, Kanazawa A, Kurokawa M, Saito M, Harada R, Kobayashi H, Ito H, Uno T.
  • Journal Title: Journal of Radiation Research Volume: 55 Pages: 996-1001
  • Peer Reviewed
• [Journal Article] Is the Outcome of a Salvage Surgery for T4 Thoracic Esophageal Squamous Cell Carcinoma Really Poor? (2014)
  • Author(s): Akutsu Y, Kono T, Uesato M, Hoshino I, Murakami K, Aoyagi T, Ota T, Toyozumi T, Suito H, Kobayashi H, Harada R, Uno T, Matsubara H.
  • Journal Title: World Journal of Surgery Volume: 38(11) Pages: 2891-7
  • Peer Reviewed
• [Journal Article] Coronal reformatted CT images contribute to the precise evaluation of the radiofrequency ablative margin for hepatocellular carcinoma (2014)
  • Author(s): Motoyama T, Ogasawara S, Chiba T, Higashide T, Yokota H, Kanogawa N, Suzuki E, Ooka Y, Tawada A, Irie R, Ochi S, Masuda Y, Uno T, Yokosuka O.
  • Journal Title: Abdom Imaging Volume: 39 Pages: 262-8
  • Peer Reviewed
• [Journal Article] Increased risk of gastric adenocarcinoma after treatment of primary gastric diffuse large B-cell lymphoma. (2013)
  • Author(s): Inaba K, Kushima R, Murakami N, Kuroda Y, Harada K, Kitaguchi M, Yoshio K, Sekii S, Takahashi K, Morota M, Mayahara H, Ito Y, Sumi M, Uno T, Itami J.
  • Journal Title: BMC Cancer Volume: 13 Pages: 499-499
  • Peer Reviewed
• [Journal Article] Patterns of radiotherapy practice for biliary tract cancer in Japan: results of the Japanese radiation oncology study group (JROSG) survey. (2013)
  • Author(s): Isohashi F, Ogawa K, Oikawa H, Onishi H, Uchida N, Maebayashi T, Kanesaka N, Tamamoto T, Asakura H, Kosugi T, Uno T, Ito Y, Karasawa K, Takayama M, Manabe Y, Yamazaki H, Takemoto M, Yoshioka Y, Nemoto K, Nishimura Y
  • Journal Title: Radiat Oncol. Volume: 8 Pages: 76-76
  • Peer Reviewed
• [Presentation] Failure pattern in patients with intermediate to high risk cervical cancer treated with postoperative chemoradiation using CT-based target delineation. (2013)
  • Author(s): Uno T, Watanabe-Nemoto M, Mitsuhashi A, Harada R, Togasaki G, Kanazawa A, Shozu M, Isobe K.
  • Organizer: ASTRO 55th Annual Meeting
  • Place of Presentation: Atlanta, GA, U.S.A.
• [Book] 放射線生物実験3.1、新・医用放射線技術実験 基礎編 (2016)
  • Author(s): 斉藤正好、 田中 仁、山田勝彦、安部真治 編
  • Total Pages: 456
  • Publisher: 共立出版
• [Book] 食道癌11、放射線療法 (2015)
  • Author(s): 宇野 隆、小澤壮治、木下芳一 編
  • Publisher: 南江堂