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Metabolome analysis of invaded pancreatic cancer cell line, PANC-1, after irradiation

Research Project

Project/Area Number 25461934
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Radiation science
Research InstitutionNational Institute of Radiological Sciences

Principal Investigator

Imai Takashi  国立研究開発法人放射線医学総合研究所, 重粒子医科学センター, プログラムリーダー (50183009)

Co-Investigator(Renkei-kenkyūsha) Ishikawa Atsuko  国立研究開発法人放射線医学総合研究所, 重粒子医科学センター, 主任技術員 (30443063)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords転移 / 浸潤 / 粒子線治療 / メタボローム解析 / 代謝経路 / がん / 放射線誘導 / メタボローム / グルタチオン / 活性酸素 / 放射線治療 / PANC-1細胞 / 一酸化窒素
Outline of Final Research Achievements

The aim of this study is to identify the metabolic pathways characterized in the invaded cancer cells, PANC-1, whose invasiveness is enhanced after the carbon-ion (C-ion) irradiation, and propose the new candidates reducing the C-ion enhanced invasiveness. Metabolome analysis using CF-TOFMS showed that several metabolites in the glycolytic pathway, the uric acid pathway synthesizing arginine, and the folate pathway producing glutathione were all increased in the invaded PANC-1 cells compared to those of whole-cultured PANC-1 cells. Such increases in the metabolite were also shown in the invaded cells after the C-ion irradiation. From the results, we next clarified the enzymes responsible for the synthesis of increased metabolites found in the invaded cells. This approach would be useful for identifying new inhibitors for the invasion of cancer cells.

Report

(5 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Products Report
  • Research Products

    (9 results)

All 2017 2015 2014 Other

All Journal Article (3 results) (of which Peer Reviewed: 3 results,  Open Access: 3 results,  Acknowledgement Compliant: 2 results) Presentation (4 results) (of which Invited: 1 results) Remarks (2 results)

  • [Journal Article] Metabolic characterization of invaded cells of the pancreatic cancer cell line, PANC-1.2017

    • Author(s)
      Mayumi Fujita, Kaori Imadome, Takashi Imai
    • Journal Title

      Cancer science

      Volume: in press Issue: 5 Pages: 961-971

    • DOI

      10.1111/cas.13220

    • Related Report
      Products Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Carbon-ion irradiation suppresses migration and invasiveness of human pancreatic carcinoma cells MIAPaCa-2 via Rac1 and RhoA degradation2015

    • Author(s)
      Fujita, M., Imadome, K., Shoji, Y., Isozaki, T., Endo, S., Yamada, S., and Imai, T.
    • Journal Title

      International Journal of Radiation Oncology Biology Physics

      Volume: 未確定 Issue: 1 Pages: 173-180

    • DOI

      10.1016/j.ijrobp.2015.05.009

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Nitric oxide increase the invasion of pancreatic cancer cells via activation of the PI3K-AKT and RhoA pathways after carbon ion irradiation2014

    • Author(s)
      Fujita, M., Imadome, K., Endo, S., Shoji, Y., Yamada, S., and Imai, T.
    • Journal Title

      FEBS Letters

      Volume: 588 Issue: 17 Pages: 3240-3250

    • DOI

      10.1016/j.febslet.2014.07.006

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] Role of nitric oxide in invasiveness of tumor cells irradiated with carbon-ion beams2015

    • Author(s)
      Mayumi Fujita1, Kaori IFujita, M, Imadome, K, Shoji, Y, Cheng, R, Kesarwala, AK, Wink, DA, and Imai, T
    • Organizer
      American Association for Cancer Research
    • Place of Presentation
      Philadelphia, Pennsylvania, USA
    • Year and Date
      2015-04-18 – 2015-04-22
    • Related Report
      2014 Research-status Report
  • [Presentation] 炭素線照射はGTP-bound Rac1 とRhoA のタンパク分解を介し膵癌由来細胞株MIAPaCa-2 の浸潤能を抑制する2014

    • Author(s)
      藤田真由美、山田滋、今井高志
    • Organizer
      第73回日本癌学会学術総会
    • Place of Presentation
      横浜
    • Year and Date
      2014-09-25 – 2014-09-27
    • Related Report
      2014 Research-status Report
  • [Presentation] 重粒子線治療と化学療法の併用~放射線生物学的立場から2014

    • Author(s)
      今井高志
    • Organizer
      第12回日本臨床腫瘍学会学術集会
    • Place of Presentation
      福岡
    • Year and Date
      2014-07-19
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] ヒト膵癌由来細胞株PANC-1の炭素線照射による代謝変動の解析

    • Author(s)
      藤田 真由美, 今留 香織, 荘司 好美, 今井 高志
    • Organizer
      第1回がんと代謝研究会
    • Place of Presentation
      山形県、鶴岡市
    • Related Report
      2013 Research-status Report
  • [Remarks] 放射線医学総合研究所先端粒子線生物研究プログラムホームページ

    • URL

      http://133.63.22.22/radgenomics/index.php

    • Related Report
      2014 Research-status Report
  • [Remarks] 放射線医学総合研究所先端粒子線生物研究プログラムホームページ

    • URL

      http://133.63.22.22/radgenomics/index.php

    • Related Report
      2013 Research-status Report

URL: 

Published: 2014-07-25   Modified: 2019-07-29  

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