| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Outline of Final Research Achievements |
CTOS (cancer tissue-originated spheroid) from colorectal cancer patients entered a reversible dormant status when cultured in hypoxia and growth factor deprived conditions. CTOS retained the characteristics of highly differentiated adenocarcinoma, and the expression of stemness or differentiation genes changed drastically depending on the culture conditions.
In the present study, we established a radiation sensitivity assay using colon cancer CTOS. CTOS in dormant status showed radiation resistance compared to the normal culture conditions, and dormant CTOS showed high expression of stemness and differentiated genes at the same time. In addition to the well-known radio-resistant mechanism, such as cell cycle arrest and hypoxia, dormant CTOS might gain the radiation resistance through the fluctuation of stemness and differentiation.
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