Comprehensive characterization of next generation strategy to improve clinical islet transplantation

• Project/Area Number: 25461946
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: General surgery
• Research Institution: Osaka University
• Principal Investigator: Kawamoto Koichi 大阪大学, 医学(系)研究科(研究院), 特任助教(常勤) (30432470)
• Co-Investigator(Kenkyū-buntansha): 和田 浩志 大阪大学, 医学(系)研究科(研究院), 助教 (00572554) ; 濱 直樹 独立行政法人国立病院機構大阪医療センター(臨床研究センター), その他部局等, 研究員 (00645723) ; 永野 浩昭 山口大学, 医学(系)研究科(研究院), 教授 (10294050) ; 小林 省吾 地方独立行政法人大阪府立病院機構大阪府立成人病センター(研究所), その他部局等, その他 (30452436) ; 秋田 裕史 地方独立行政法人大阪府立病院機構大阪府立成人病センター(研究所), その他部局等, その他 (70528463) ; 江口 英利 大阪大学, 医学(系)研究科(研究院), 准教授 (90542118)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 膵島 / β細胞 / 再生医療 / 移植 / 膵島移植 / 組織移植 / 膵β細胞 / ADSC / 条件培地 / BETA2/NeuroD1 / インスリン / 幹細胞 / 共移植 / 糖尿病

Outline of Final Research Achievements

In the current study, we have demonstrated that immunosuppressants, which induce autophagy, might be avoided. Furthermore, pancreatic niche formation like liver niche would be good option for both islet transplantation and stem cell differentiation. The use of conditioned medium could improve beta cell differentiation.

Research Products

• [Journal Article] Fetal Hepatocyte Derived Culture Medium Elicits Adipocyte Differentiation to Bile Duct Cell Lineages in Mouse (2016)
  • Author(s): Ogawa H, Konno M, Kawamoto K, Nishida N, Koseki J, Mizushima T, Satoh T, Eguchi H, Doki Y, Mori M, Ishii H
  • Journal Title: Biomed Rep Volume: In press
  • Peer Reviewed
• [Journal Article] A Trans-omics mathematical analysis reveals novel functions of ornithine metabolic pathway in cancer stem cells (2016)
  • Author(s): Koseki J, Matsui H, Konno M, Nishida N, Kawamoto K, Kano Y, Mori M, Doki Y, Ishii H，
  • Journal Title: Sci Rep Volume: 6 Pages: 20726-20726
  • Peer Reviewed
• [Journal Article] Murine Insulinoma Cell-Conditioned Medium with ΒETA2/Neurod1 Transduction Efficiently Induces the Differentiation of Adipose-Derived Mesenchymal Stem Cells into β-Like Cells both In Vitro and In Vivo (2014)
  • Author(s): Koichi Kawamoto
  • Journal Title: Journal of Stem Cell Research & Therapy Volume: 4 Issue: 08 Pages: 221-230
  • DOI: 10.4172/2157-7633.1000221
  • Peer Reviewed
• [Journal Article] CD90 (Thy-1)-high selection enhances reprograming capacity of murine adipose-derived stem cells. (2013)
  • Author(s): Kawamoto, K., Konno, M., Nagano, H., Nishikawa, S., Tomimaru, Y., Akita, H., Hama, N., Wada, H., Kobayashi, S., Eguchi, H., Ito, T., Doki, Y., Mori, M., Ishii, H.
  • Journal Title: Dis Markers. Volume: 35 Pages: 573-579
  • DOI: 10.1155/2013/392578
  • Peer Reviewed
• [Journal Article] Adipose-derived mesenchymal stem cells and regenerative medicine. (2013)
  • Author(s): Konno, M., Hamabe, A., Hasegawa, S., Ogawa, H., Fukusumi, T., Nishikawa, S., Ohta, K., Kano, Y., Ozaki, M., Noguchi, Y., Sakai, D., Kudoh, T., Kawamoto, K., Eguchi, H., Satoh, T., Tanemura, M., Nagano, H., Doki, Y., Mori, M., Ishii, H.
  • Journal Title: Dev. Growth Differ. Volume: 55 Issue: 3 Pages: 309-318
  • DOI: 10.1111/dgd.12049
  • Peer Reviewed
• [Presentation] Murine insulinoma cell-conditioned medium with ΒETA2/NeuroD1 transduction efficiently induces the differentiation of adipose-derived mesenchymal stem cells into β-like cells both in vitro and in vivo (2014)
  • Author(s): Koichi Kawamoto
  • Organizer: Cell Symposia, Stem Cell Energetics
  • Place of Presentation: Berkeley, CA, USA
  • Year and Date: 2014-12-09 – 2014-12-11
• [Presentation] 臨床膵島移植成績の改善を目指した基礎研究-共移植モデルと新規β細胞誘導法の効果について- (2014)
  • Author(s): 川本 弘一
  • Organizer: 第41回日本臓器保存生物医学会学術集会（奨励賞受賞記念講演）
  • Place of Presentation: 千里ライフサイエンスセンター（大阪）
  • Year and Date: 2014-11-28 – 2014-11-29
• [Presentation] Islet Cell Biology, Regeneration, and Transplantation (2014)
  • Author(s): Koichi Kawamoto
  • Organizer: The Qatar-Japan Symposium on Tissue Regeneration Therapy, Invited Lecture
  • Place of Presentation: Doha, Qatar
  • Invited
• [Presentation] Pancreas transplantation using old donor organs in Japan (2013)
  • Author(s): Koichi Kawamoto
  • Organizer: The 13th Congress of the Asian Society of Transplantation (CAST2013)
  • Place of Presentation: Kyoto
• [Presentation] 細胞療法における CD90high ADSC 亜分画の有効性についての解析 (2013)
  • Author(s): 川本 弘一
  • Organizer: 第40回日本臓器保存生物医学会学術集会
  • Place of Presentation: 新宿
• [Presentation] 提供された膵臓を有効に移植するには? 高齢ドナーからの膵臓移植の可能性 (2013)
  • Author(s): 川本 弘一
  • Organizer: 第49回日本移植学会
  • Place of Presentation: 京都