The role of Sonic hedgehog in intestinal injury via stem cell

• Project/Area Number: 25461951
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: General surgery
• Research Institution: The University of Tokushima
• Principal Investigator: YOSHIKAWA Kozo 徳島大学, 大学病院, 助教 (80448331)
• Co-Investigator(Kenkyū-buntansha): BANDO Yoshimi 徳島大学, 病院, 准教授 (00238239) ; KURITA Nobuhiro 徳島大学, 病院, 特任教授 (30335814) ; SATO Hirohiko 徳島大学, 病院, 特任助教 (80609694)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 腸管修復 / Sonic hedgehog / 幹細胞 / 腸管障害 / Shh pathway / Cancer stem cell

Outline of Final Research Achievements

This study focused on the relationship between the intestinal injury and Sonic hedgehog pathway. In intestinal injury models, Shh pathway was downregulated. HCT-116 sphere-derived cells showed significantly increased expression of stem cell markers, Shh downstream genes and epithelial-mesenchymal transition (EMT) markers compared to parental cells grown in conventional culture. The expression of stemness markers, Shh downstream genes and EMT markers were higher in cancer spheres than the parental cell line and downregulated by cyclopamine treatment in a dose-dependent manner. Shh pathway is related with cancer stem cell and may have an important role in intestinal injury.

Research Products

• [Journal Article] Combined liver mobilization and retraction: A novel technique to obtain the optimal surgical field during laparoscopic total gastrectomy. (2016)
  • Author(s): Yoshikawa K, Shimada M, Higashijima J, Nakao T, Nishi M, Takasu C, Kashihara H, Eto S.
  • Journal Title: Asian Journal of Endoscopic Surgery Volume: 印刷中 Pages: 111-115
  • DOI: 10.1111/ases.12271
  • Peer Reviewed
• [Journal Article] Effect of Daikenchuto, a Traditional Japanese Herbal Medicine, after Total Gastrectomy for Gastric Cancer: A Multicenter, Randomized, Double-Blind, Placebo-Controlled, Phase II Trial. (2015)
  • Author(s): Yoshikawa K, Shimada M, Wakabayashi G, Ishida K, Kaiho T, Kitagawa Y
  • Journal Title: J Am Coll Surg Volume: 221 Pages: 571-578
  • DOI: 10.1016/j.jamcollsurg.2015.03.004
  • Peer Reviewed
• [Journal Article] Cyclopamine decreased the expression of Sonic Hedgehog and its downstream genes in colon cancer stem cells. (2014)
  • Author(s): Batsaikhan BE, Yoshikawa K, Kurita N, Iwata T, Takasu C, Kashihara H, Shimada M
  • Journal Title: Anticancer Res Volume: 34 Pages: 6339-6344
  • Peer Reviewed
• [Journal Article] Characteristics of internal hernia after gastrectomy with Roux-en-Y reconstruction for gastric cancer. (2014)
  • Author(s): Yoshikawa K, Shimada M, Kurita N, Sato H, Iwata T, Higashijima J, Chikakiyo M, Nishi M, Kashihara H, Takasu C, Matsumoto N, Eto S
  • Journal Title: Surg Endosc Volume: 印刷中 Pages: 1774-1778
  • DOI: 10.1007/s00464-013-3384-7
  • Peer Reviewed
• [Journal Article] Hybrid technique for laparoscopic incisional ventral hernia repair combining laparoscopic primary closure and mesh repair. (2014)
  • Author(s): Yoshikawa K, Shimada M, Kurita N, Sato H, Iwata T, Higashijima J, Chikakiyo M, Nishi M, Kashihara H, Takasu C, Matsumoto N, Eto S
  • Journal Title: Asian J Endosc Surg. Volume: 7 Pages: 282-285
  • DOI: 10.1111/ases.12113
  • Peer Reviewed
• [Presentation] がん幹細胞制御におけるSonic Hedgehog signalingの役割 (2015)
  • Author(s): 中尾 寿宏
  • Organizer: 第101回日本消化器病学会
  • Place of Presentation: 仙台国際センター（宮城県仙台市）
  • Year and Date: 2015-04-23
• [Presentation] 癌幹細胞制御におけるSonic Hedgehog signalingの役割 (2014)
  • Author(s): 東島潤, 島田光生, 栗田信浩, 佐藤宏彦, 岩田貴, 吉川幸造, 近清素也, 西正暁, 柏原秀也, 松本規子
  • Organizer: 第100回日本消化器病学会総会
  • Place of Presentation: 東京国際フォーラム(東京都千代田区)
  • Year and Date: 2014-04-23 – 2014-04-26
• [Presentation] ヒト脂肪由来幹細胞による肝傷害軽減効果に関する基礎的検討 (2013)
  • Author(s): 齋藤裕, 島田光生, 宇都宮徹, 池本哲也, 山田眞一郎, 石川大地, 岩橋衆一, 金本真美, 荒川悠佑, 森大樹, 森根裕二, 居村暁
  • Organizer: 第49回 日本移植学会総会
  • Place of Presentation: 国立京都国際会館(京都府)
• [Presentation] 脂肪由来幹細胞の傷害肝へのホーミング効果―癌幹細胞転移メカニズムへの応用― (2013)
  • Author(s): 齋藤裕, 島田光生, 宇都宮徹, 池本哲也, 山田眞一郎, 森根裕二, 居村暁, 森大樹, 荒川悠佑, 金本真美, 岩橋衆一, 髙須千絵,
  • Organizer: 第72回 日本癌学会学術総会
  • Place of Presentation: パシフィコ横浜(神奈川県)
• [Presentation] 癌幹細胞の治療抵抗性獲得におけるepigenetic修飾の意義 (2013)
  • Author(s): 岩橋衆一, 島田光生, 宇都宮徹, 居村暁, 森根裕二, 池本哲也, 荒川悠佑, 金本真美, 齋藤裕, 石川大地
  • Organizer: 第72回 日本癌学会学術総会
  • Place of Presentation: パシフィコ横浜(神奈川県)