Novel spatiotemporal analysis of mechanism for rejection after liver transplantation using two-photon microscopy
| Project/Area Number |
25461954
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General surgery
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| Research Institution | Kumamoto University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
INOMATA Yukihiro 熊本大学, 大学院生命科学研究部, 教授 (50193628)
HONDA Masaki 熊本大学, 大学院生命科学研究部, 助教 (80573609)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | マウス同所性肝移植 / ラット同所性肝移植 / 肝虚血再灌流障害 / 好中球動態 / 2光子励起顕微鏡 / 急性拒絶反応 |
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| Outline of Final Research Achievements |
We showed a novel method for examining neutrophil recruitment in liver transplant model and hepatic ischemia-reperfusion(I/R) injury model using two-photon microscopy.
In liver transplant model, we could observe the blood circulation in sinusoids and the behavior of neutrophils.
In I/R model, at low magnification, four to six hepatic lobules could be visualized. The number of adherent neutrophils continued to increase for 4 hr after reperfusion, whereas their crawling velocity reached a maximum of 2 hr after reperfusion and then decreased gradually. High-magnification images revealed the presence or absence of blood circulation in sinusoids. Adherent neutrophils in perfused areas in the I/R group had more elongated shapes and moved more quickly than those in nonperfused areas and in the control group.
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Report
(4 results)
Research Products
(2 results)
