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Novel spatiotemporal analysis of mechanism for rejection after liver transplantation using two-photon microscopy

Research Project

Project/Area Number 25461954
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field General surgery
Research InstitutionKumamoto University

Principal Investigator

takeichi takayuki  熊本大学, 医学部附属病院, 非常勤診療医師 (00380999)

Co-Investigator(Kenkyū-buntansha) INOMATA Yukihiro  熊本大学, 大学院生命科学研究部, 教授 (50193628)
HONDA Masaki  熊本大学, 大学院生命科学研究部, 助教 (80573609)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywordsマウス同所性肝移植 / ラット同所性肝移植 / 肝虚血再灌流障害 / 好中球動態 / 2光子励起顕微鏡 / 急性拒絶反応
Outline of Final Research Achievements

We showed a novel method for examining neutrophil recruitment in liver transplant model and hepatic ischemia-reperfusion(I/R) injury model using two-photon microscopy.
In liver transplant model, we could observe the blood circulation in sinusoids and the behavior of neutrophils.
In I/R model, at low magnification, four to six hepatic lobules could be visualized. The number of adherent neutrophils continued to increase for 4 hr after reperfusion, whereas their crawling velocity reached a maximum of 2 hr after reperfusion and then decreased gradually. High-magnification images revealed the presence or absence of blood circulation in sinusoids. Adherent neutrophils in perfused areas in the I/R group had more elongated shapes and moved more quickly than those in nonperfused areas and in the control group.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (2 results)

All 2013

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (1 results)

  • [Journal Article] Intravital imaging of neutrophil recruitment in hepatic ischemia-reperfusion injury in mice.2013

    • Author(s)
      Honda M, Takeichi T, Asonuma K, Tanaka K, Kusunoki M, Inomata Y.
    • Journal Title

      Transplantation

      Volume: 95 Issue: 4 Pages: 551-558

    • DOI

      10.1097/tp.0b013e31827d62b5

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] Intravital Two-Photon Imaging of Neutrophil Recruitment Reveals an Efficacy of FPR1 Blockade to Attenuate the Hepatic Ischemia-Reperfusion Injury2013

    • Author(s)
      Masaki Honda
    • Organizer
      ATC(American Transplant Congress)
    • Place of Presentation
      Washington State Convention & Trade Center(USA、seattle)
    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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