Role of mebrane transporters in drug resistance of pancreatic cancer cells
Project/Area Number |
25461956
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
General surgery
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Research Institution | Yokohama City University |
Principal Investigator |
UENO Yasuharu 横浜市立大学, 医学部, 助教 (60375235)
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Project Period (FY) |
2013-04-01 – 2016-03-31
|
Project Status |
Completed (Fiscal Year 2015)
|
Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
|
Keywords | 癌幹細胞 / 膵癌 / プロテオーム解析 / プロテオーム / トランスポーター |
Outline of Final Research Achievements |
The prognosis of pancreatic cancer remains very poor, the mainly reason might be the prominent resistance to chemoradiotherapy. However, the mechanism of chemoradiotherapy resistance in pancreatic cancer has not been fully understood. We aim to investigate the relationship between pancreatic cancer stem cells and chemoradiotherapy resistance, and identify the specific biomarkers, which promote tumor survival in pancreatic cancer with chemoradiotherapy. Firstly, we transplanted human pancreatic cancer tissue into mice to construct pancreatic cancer mice model. With gemcitabine treatment, we found that the population of pancreatic cancer stem cells was increasing. Moreover, we identified expression of multiple transporters were upregulated after gemcitabine treatment with proteomic analysis, and these upregulated transporters were mainly detected in CD133+CD44+ cancer cells. Our results suggest that these transporters may exhibit important roles in the chemoradiotherapy resistance.
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Report
(4 results)
Research Products
(17 results)
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[Journal Article] Vascularized and Complex Organ Buds from Diverse Tissues via Mesenchymal Cell-Driven Condensation2015
Author(s)
T. Takebe, M. Eomura, E. Yoshizawa, M. Kimura, H. Koike, Y. Ueno, T. Matsuzaki, T. Yamazaki, T. Toyohara, K. Osafune, H. Nakauchi, H. Y. Yoshikawa, H. Taniguchi
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Journal Title
Cell Stem Cell
Volume: 16
Issue: 5
Pages: 556-565
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Polycomb group protein Ezh2 regulates hepatic progenitor cell proliferation and differentiation in murine embryonic liver.2014
Author(s)
Koike H, Ouchi R, Ueno Y, Nakata S, Obana Y, Sekine K, Zheng YW, Takebe T, Isono K, Koseki H, Taniguchi H.
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Journal Title
PLoS One.
Volume: 9
Issue: 8
Pages: 0-2
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] The evidence of sophisticatedly heterogeneous population of human umbilical vein endothelial cells2014
Author(s)
Zheng YW, Nie YZ, Tsuchida T, Zhang RR, Aoki K, Sekine K, Ogawa M, Takebe T, Ueno Y, Sakakibara H, Hirahara F, Taniguchi H
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Journal Title
Transplant Proc
Volume: 46
Issue: 4
Pages: 1251-3
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] The CD133+CD44+ Precancerous Subpopulation of Oval Cells is a Therapeutic Target for Hepatocellular Carcinoma2014
Author(s)
Zheng YW, Tsuchida T, Shimao T, Li B, Takebe T, Zhang RR, Sakurai Y, Ueno Y, Sekine K, Ishibashi N, Imajima M, Tanaka T, and Taniguchi H
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Journal Title
Stem Cells Dev
Volume: (In press)
Issue: 18
Pages: 2237-2249
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Ring1B Promotes Hepatic Stem/Progenitor Cell Expansion via Simultaneous Suppression of Cdkn1a and Cdkn2a.2014
Author(s)
Koike H, Ueno Y, Naito T, Shiina T, Nakata S, Ouchi R, Obana Y, Sekine K, Zheng YW, Takebe T, Isono K, Koseki H, Taniguchi H
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Journal Title
Hepatology
Volume: 印刷中
Issue: 1
Pages: 323-333
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Vascularized and functional human liver from an iPSC-derived organ bud transplant.2013
Author(s)
Takanori Takebe*, Keisuke Sekine, Masahiro Enomura, Hiroyuki Koike, Ran-Ran Zhang, Yasuharu Ueno, Yun-Wen Zheng, Naoto Koike, Shinsuke Aoyama, Yasuhisa Adachi, Hideki Taniguch*(*: Correspondence)
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Journal Title
Nature
Volume: 499
Issue: 7459
Pages: 481-484
DOI
NAID
Related Report
Peer Reviewed
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[Presentation] Targeted proteomic absolute quantification on transporters of human pancreatic cancer cells with gemcitabine-resistance2013
Author(s)
Yasuharu Ueno, Sayuri Hoshino, Nana Tsuchida, Susumu Nakata, Yasuo Uchida, Keisuke Sekine, Yun-Wen Zheng, Masanao Kurata, Soichiro Morinaga, Yohei Miyagi, Tomoyuki Yokose, Itaru Endo, Tetsuya Terasaki, Hisashi Hirano, Hideki Taniguchi
Organizer
12th Human Proteome Organization World Congress
Place of Presentation
パシフィコ横浜(神奈川県横浜市)
Related Report
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[Presentation] Analysis of chemoradiotherapy resistance in the human pancreatic cancer stem cells2013
Author(s)
Sayuri Hoshino, Keisuke Sekine, Lue Sun, Susumu Nakata, Tetsuya Terasaki, Souichiro Morinaga, Youhei Miyagi, Itaru Endo, Tomoyuki Yokose, Masanao Kurata, Yasuharu Ueno, Hideki Taniguchi
Organizer
72nd Annual Meeting of the Japanese Cancer Association
Place of Presentation
パシフィコ横浜(神奈川県横浜市)
Related Report
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