• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Immunotolerance induction in kidney transplantation by infusion of regulatory cells

Research Project

Project/Area Number 25461964
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field General surgery
Research InstitutionTokyo Women's Medical University

Principal Investigator

Koyama Ichiro  東京女子医科大学, 医学部, 助教 (10266760)

Co-Investigator(Kenkyū-buntansha) FUCHINOUE Shohei  東京女子医科大学, 医学部, 准教授 (10147382)
TERAOKA Satoshi  国際医療福祉大学, 大学病院, 教授 (20147383)
BASHUDA Hisashi  順天堂大学, 医学部, 助教 (40439295)
OKUMURA Ko  順天堂大学, 医学部, 教授 (50009700)
MURAKAMI Toru  東京女子医科大学, 医学部, 助教 (50287356)
NAKAJIMA Ichiro  東京女子医科大学, 医学部, 講師 (80198077)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords腎臓移植 / 免疫寛容 / 移植免疫寛容 / 臓器移植
Outline of Final Research Achievements

This study was performed in kidney transplant (KTx) recipients from living donors. Lymphocytes were harvested 2 days before KTx. Recipient's PBMCs were co-cultured with irradiated (30Gy) donor cells in the presence of anti CD80/CD86 mAb for 2 weeks. As preconditioning, splenectomy or rituximab + cyclophosphamide (CP) were employed. Twelve days after KTx, the cultured cells were intravenously infused, after that immunosuppressants were gradually tapered. In the cultured cells, CD4, CD25, and FoxP3 expressions were upregulated. The recipients showed excellent graft survival rate and function. Final doses of CsA and MMF were almost half of conventional KTx, however high incidence of BPAR: 3 subclinical, 4 anti-rejection treatments, obliged us to discontinue further reducing immunosuppressants. These results showed that adoptive transfer of anergic cells could be one of promising approaches as tolerance-inducing strategy.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (3 results)

All 2016 2013

All Journal Article (1 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 1 results,  Open Access: 1 results) Presentation (2 results)

  • [Journal Article] A pilot study of operational tolerance with a regulatory T cell-based cell therapy in living donor liver transplantation.2016

    • Author(s)
      ①Todo S, Yamashita K, Goto R, Zaitsu M, Nagatsu A, Oura T, Watanabe M, Aoyagi T, Suzuki T, Shimamura T, Kamiyama T, Sato N, Sugita J, Hatanaka K, Bashuda H, Habu S, Demetris AJ, Okumura K
    • Journal Title

      Hepatology

      Volume: 00 Issue: 2 Pages: 00-00

    • DOI

      10.1002/hep.28459

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] Donor specific hyporesponsiveness in kidney transplantation using adoptive transfer of Ex Vivo induced tolerogenic cells2013

    • Author(s)
      小山一郎
    • Organizer
      American Transplant Congress
    • Place of Presentation
      シアトル
    • Related Report
      2013 Research-status Report
  • [Presentation] Donor specific hyporesponsiveness in kidney transplantation using adoptive transfer of Ex Vivo induced tolerogenic cells2013

    • Author(s)
      小山一郎
    • Organizer
      International Xenotransplantation Association
    • Place of Presentation
      大阪
    • Related Report
      2013 Research-status Report

URL: 

Published: 2014-07-25   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi