| Project/Area Number |
25461975
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General surgery
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| Research Institution | University of Toyama |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
TSUKADA Kazuhiro 富山大学, 大学院医学薬学研究部(医学), 教授 (90171967)
SHIMADA Yutaka 富山大学, 大学院医学薬学研究部(医学), 准教授 (30216072)
OZAWA Tatsuhiko 富山大学, 大学院医学薬学研究部(免疫学), 助教 (10432105)
吉田 徹 富山大学, 大学病院, 助教 (40456364)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | TNBC / CTC / KLF4 / siRNA / EMT / OS / DFS / 乳癌 / トリプルネガティブ / NANOG / 培養細胞株 |
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| Outline of Final Research Achievements |
We assessed the expression levels of KLF4 in 84 patients with TNBC by immunohistochemical staining. In addition, CTCs in the peripheral blood of TNBC patients were identified and compared with primary lesions in terms of KLF4 expression. Moreover, the expression of KLF4 was inhibited by transfecting cultured TNBC cells with the siRNA of KLF4 to analyze the effects of KLF4 on cell proliferation and EMT-like changes.
In the 84 patients with TNBC, higher KLF4 expression was associated with significantly better OS and DFS. KLF4 expression was lower in CTCs than in cancer cells in TNBC primary lesions. TNBC cells with inhibited KLF4 expression exhibited a greater ability to growth. These cells also underwent EMT-like changes with reduced expression of epitherial factors such as E-cadherin.
TNBC patients with reduced KLF4 expression had poor outcomes. The results of our experiments suggest the expression of KLF4 is one of the important factors that inhibit the EMT and growth of TNBC.
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