Development of a prediction model for lymph node metastasis in luminal A subtype breast cancer

• Project/Area Number: 25461981
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: General surgery
• Research Institution: Osaka University
• Principal Investigator: Naoi Yasuto 大阪大学, 医学(系)研究科(研究院), 助教 (30646211)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 乳癌 / マイクロアレイ / 多重遺伝子診断 / 腋窩リンパ節転移 / 腋窩リンパ節 / 転移予測

Outline of Final Research Achievements

The present study aimed to construct a prediction model for axillary lymph node metastasis (ALNM) using a DNA microarray assay for gene expression in breast tumor tissues. Luminal A breast cancers, diagnosed by PAM50 testing, were analyzed, and a prediction model (genomic nodal index (GNI)) consisting of 292 probe sets for ALNM was constructed in a training set of patients (n = 388), and was validated in the first (n = 59) and the second (n = 103) validation sets. AUCs of ROC were 0.820, 0.717, and 0.749 in the training, first, and second validation sets, respectively. GNI was most significantly associated with ALNM, independently of the other conventional clinicopathological parameters in all cohorts. It is suggested that GNI can be used to identify the patients with a low risk for ALNM so that sentinel lymph node biopsy can be spared safely.

Research Products

• [Journal Article] Development of a prediction model for lymph node metastasis in luminal A subtype breast cancer: the possibility to omit sentinel lymph node biopsy. (2014)
  • Author(s): Nakauchi C, Naoi Y, Shimazu K, Tsunashima R, Nishio M, Maruyama N, Shimomura A, Kagara N, Shimoda M, Kim SJ, Noguchi S.
  • Journal Title: Cancer Lett. Volume: Oct 10;353(1) Issue: 1 Pages: 52-8
  • DOI: 10.1016/j.canlet.2014.07.003
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] 72-gene classifier for predicting prognosis of estrogen receptor-positive and node-negative breast cancer patients using formalin-fixed, paraffin-embedded tumor tissues. (2014)
  • Author(s): Nishio M, Naoi Y, Tsunashima R, Nakauchi C, Kagara N, Shimoda M, Shimomura A, Maruyama N, Shimazu K, Kim SJ, Noguchi S.
  • Journal Title: Clin Breast Cancer Volume: Jun;14(3) Issue: 3 Pages: e73-e80
  • DOI: 10.1016/j.clbc.2013.11.006
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Construction of novel immune-related signature for prediction of pathological complete response to neoadjuvant chemotherapy in human breast cancer. (2014)
  • Author(s): Sota Y, Naoi Y, Tsunashima R, Kagara N, Shimazu K, Maruyama N, Shimomura A, Shimoda M, Kishi K, Baba Y, Kim SJ, Noguchi S.
  • Journal Title: Ann Oncol. Volume: Jan;25(1) Issue: 1 Pages: 100-6
  • DOI: 10.1093/annonc/mdt427
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] 乳癌個別化医療を実現する分子診断の展望 (2015)
  • Author(s): 直居 靖人
  • Organizer: 日本病理学会総会
  • Place of Presentation: 名古屋国際会議場（名古屋市）
  • Year and Date: 2015-05-01
  • Invited
• [Presentation] 疾患マーカーの探索と利用 (2014)
  • Author(s): 直居靖人
  • Organizer: 第87回 日本生化学会大会 フォーラム 招待講演，2F07
  • Place of Presentation: 国立京都国際会館
  • Year and Date: 2014-10-15 – 2014-10-18
  • Invited
• [Presentation] 乳癌新規バイオマーカー ～予後予測から感受性予測まで (2014)
  • Author(s): 直居靖人
  • Organizer: Osaka breast cancer workshop 2014 招待講演
  • Place of Presentation: 大阪
  • Year and Date: 2014-10-15
  • Invited
• [Presentation] マイクロアレイによる乳癌の新分類学 (2014)
  • Author(s): 直居靖人
  • Organizer: 第26回 大阪若手がんセミナー 招待講演
  • Place of Presentation: 大阪
  • Year and Date: 2014-07-15
  • Invited
• [Presentation] 95GC as a Predictor of Recurrence Risk for ER+/Node- Breast Cancer Patients (2014)
  • Author(s): 直居靖人
  • Organizer: 第114回 日本外科学会定期学術集会 ワークショップ 招待講演
  • Place of Presentation: 国立京都国際会館
  • Year and Date: 2014-04-03 – 2014-04-05
  • Invited