The Novel Comprehensive Screening of Anticancer Drug Resistant Genes of Breast Cancer Cell
| Project/Area Number |
25461997
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General surgery
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| Research Institution | Keio University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
Hayashida Tetsu 慶應義塾大学, 医学部, 講師 (80327543)
Jinno Hiromitsu 帝京大学, 医学部, 教授 (20216261)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 薬剤耐性 / スクリーニング / トランスポゾン / 乳癌 / 抗がん剤 / 耐性化 / 抗癌剤耐性 / 固形癌 |
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| Outline of Final Research Achievements |
Drug resistance is one of the major issues in managing esophageal cancer patients as chemotherapy is a pillar treatment today. Since drug resistance may be mediated by genetic changes in the tumor, the identification of gene mutation may lead to better outcomes in therapy. We performed a novel method using transposon for a comprehensive screening with a view to identify drug resistant genes. Transposons are DNA sequences that move from one location on the genome to another. By this nature, a modified piggyBac transposon was designed as an insertion mutagen. A cytomegalovirus (CMV) promoter sequence was added to initiate strong transcription. When the transposon is inserted to the upstream of a certain gene, the gene will be up-regulated while when inserted downstream of intragenicaly it will be down regulated. The method is inexpensive and relatively simple, and capable both in activation and disruption, leading to comprehensive screening.
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Report
(4 results)
Research Products
(3 results)
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[Journal Article] Comprehensive screening of genes resistant to an anticancer drug in esophageal squamous cell carcinoma.2015
Author(s)
Tsutsui M, Kawakubo H, Hayashida T, Fukuda K, Nakamura R, Takahashi T, Wada N, Saikawa Y, Omori T, Takeuchi H, Kitagawa Y.
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Journal Title
Int J Oncol.
Volume: 47(3)
Issue: 3
Pages: 867-74
DOI
Related Report
Peer Reviewed
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