| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2015: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Outline of Final Research Achievements |
We performed the basic study to create more safe and functional materials, which are mimicking amniotic membrane as an excellent material for regenerative medicine. In this study, we investigated the effects of anti-adhesion and peritoneal regeneration for the membrane made of alkali- or acid-treated gelatin or their complexes, containing the culture medium of stem cells established from amniotic membrane (conditioned medium: CM). The culture studies showed that CM promoted the growth of mesothelial cells and that it was more effective to condense CM at 5-10 times. From the studies using rat cecum adhesion model, the freeze dry membrane made of alkali-treated gelatin with condensed CM had most excellent anti-adhesive effect and peritoneal regeneration.
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