Development of safer and more useful materials for regenerative medicine mimicking the properties of amniotic membrane

• Project/Area Number: 25462002
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: General surgery
• Research Institution: Doshisha University
• Principal Investigator: Tsujimoto Hiroyuki 同志社大学, 研究開発推進機構, 嘱託研究員 (20521272)
• Co-Investigator(Kenkyū-buntansha): HAGIWARA AKEO 同志社大学, 生命医科学部, 教授 (90198648)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2015: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 再生医学 / 羊膜 / )再生医療材料 / 実験外科学 / 再生医療材料 / 幹細胞 / コラーゲン / ゼラチン / 足場材料 / 癒着防止剤 / 癒着防止材

Outline of Final Research Achievements

We performed the basic study to create more safe and functional materials, which are mimicking amniotic membrane as an excellent material for regenerative medicine. In this study, we investigated the effects of anti-adhesion and peritoneal regeneration for the membrane made of alkali- or acid-treated gelatin or their complexes, containing the culture medium of stem cells established from amniotic membrane (conditioned medium: CM). The culture studies showed that CM promoted the growth of mesothelial cells and that it was more effective to condense CM at 5-10 times. From the studies using rat cecum adhesion model, the freeze dry membrane made of alkali-treated gelatin with condensed CM had most excellent anti-adhesive effect and peritoneal regeneration.

Research Products

• [Journal Article] Prevention of polyglycolic acid-induced peritoneal adhesion using alginate in a rat model (2015)
  • Author(s): Matoba M, Hashimoto A, Tanzawa A, Orikasa T, Ikeda J, Iwame Y, Ozamoto Y, Abe R, Miyamoto H, Yoshida C, Hashimoto T, Torii H, Takamori H, Morita S, Tsujimoto H, Hagiwara A
  • Journal Title: BioMed Res Int Volume: 2015 Pages: 403413-403413
  • DOI: 10.1155/2015/403413
  • Peer Reviewed / Open Access
• [Journal Article] Biological properties of a thermally crosslinked gelatin film as a novel anti-adhesive material: Relationship between the biological properties and the extent of thermal crosslinking (2015)
  • Author(s): Tsujimoto H, Tanzawa A, MiyamotoH, Horii T, Ikada Y, Hagiwara A et al.
  • Journal Title: J Biomed Mater Res B Appl Biomater Volume: 103 Issue: 7 Pages: 1-8
  • DOI: 10.1002/jbm.b.33340
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Prevention of polyglycolic acid-induced peritoneal adhesion using alginate in a rat model (2015)
  • Author(s): Matoba M, Hashimoto A, Tanzawa A, Tsujimoto H, Hagiwara A et al.
  • Journal Title: Biomed Res Int Volume: 403413 Pages: 1-11
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Development of gelatin flakes, a new type of anti-adhesive meterial: a preliminary study of in vivo rat adhesion models (2014)
  • Author(s): Tsujimoto H, Takamori H, Tsujii M, Ikeda J, Orikasa T, Ikada Y, Morita S, Hagiwara A. et al.
  • Journal Title: Surg Today Volume: 44 Issue: 2 Pages: 391-394
  • DOI: 10.1007/s00595-013-0608-3
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] A preliminary study of the physical properties of a new anti-adhesive material made of thermally cross-linked gelatin film (2013)
  • Author(s): Horii T, Tsujimoto H, Miyamoto H, Nakamachi E, Hagiwara A et al.
  • Journal Title: 同志社大学 理工学研究報告 Volume: 54(1) Pages: 52-58
  • NAID: 110009575481
• [Journal Article] The effect of thermally cross-linked gelatin film on intraperitoneal dissemination of cancer cells –A In vitro study using human gastrointestinal cancer cell lines- (2013)
  • Author(s): Miyamoto H, Tsujimoto H, Horii T, Ikeda J, Orikasa T, Takamori H, Morita S, Hagiwara A et al.
  • Journal Title: 同志社大学 理工学研究報告 Volume: 54(1) Pages: 16-20
  • NAID: 110009575473
• [Journal Article] Gelatin sponge sheet combined with gelatin glue as new topical hemostatic materials -A preliminary report in an animal model- (2013)
  • Author(s): Wang Z, Kotani Y, Tamura A, TsujiM, Tsujimoto H, Hagiwara A et al.
  • Journal Title: 同志社大学 理工学研究報告 Volume: 54(1) Pages: 36-40
  • NAID: 110009575477
• [Journal Article] Non-woven PGA fabrics for the treatment of pancreatic juice leakage - A preliminary study- (2013)
  • Author(s): Kotani Y, Wang Z, Horii T, Morita S, Tsujimoto H, Hagiwara A et al.
  • Journal Title: 同志社大学 理工学研究報告 Volume: 54(1) Pages: 66-74
• [Journal Article] New scaffolds for tissue engineering using adipose-derived stem cells (2013)
  • Author(s): Kawasumi A, Wang Z, Kotani Y, Tsujimoto, Hagiwara A, et al.
  • Journal Title: 同志社大学 理工学研究報告 Volume: 54(1) Pages: 41-51
  • NAID: 110009575479
• [Presentation] 再生の足場材料を用いた新規癒着防止材熱架橋ゼラチンフィルムの物理学的・生物学的特性-HA/CMCとの比較- (2016)
  • Author(s): 堀井常人,辻本洋行,宮本博恵,山中皓暉,韓沛,阿部里恵,田中翔大,鳥井裕子,小座本雄軌,高森秀樹,筏義人,高木敏貴,萩原明郎
  • Organizer: 第15回日本再生医療学会総会
  • Place of Presentation: 大阪国際会議場(大阪市)
  • Year and Date: 2016-03-17
• [Presentation] PGA不織布のコラーゲンコーティングがADScの増殖と分化に与える影響 (2016)
  • Author(s): 宮本博恵,川澄あかり, 山中皓暉,韓沛,堀井常人,田中翔大,阿部里恵, 鳥井裕子,小座本雄軌,高森秀樹,高木敏貴,辻本洋行,萩原明郎
  • Organizer: 第15回日本再生医療学会総会
  • Place of Presentation: 大阪国際会議場(大阪市)
  • Year and Date: 2016-03-17
• [Presentation] 再生の足場材料を用いた癒着防止材 –熱架橋gelatin filmとその応用- (2015)
  • Author(s): 辻本洋行,宮本博恵,堀井常人, 山中皓暉,韓沛,阿部里恵,田中翔大,小座本雄軌,鳥井裕子,竹本洋一,鈴木周子,高森秀樹,森田真一郎,筏義人,萩原明郎
  • Organizer: 第37回日本バイオマテリアル学会
  • Place of Presentation: 京都テルサ（京都市）
  • Year and Date: 2015-11-09
• [Presentation] 再生の足場材料を用いた癒着防止材熱架橋gelatin filmの物理的・生物学的特性 –癒着防止のmechanismについて- (2015)
  • Author(s): 辻本洋行,堀井常人,宮本博恵,山中皓暉,阿部里恵,田中翔大,小座本雄軌,鳥井裕子,高森秀樹,鈴木周子,森田真一郎,筏義人,萩原明郎
  • Organizer: 第23回日本消化器関連学会週間(JDDW)
  • Place of Presentation: グランドプリンスホテル新高輪（東京都）
  • Year and Date: 2015-10-08
• [Presentation] 再生の足場材料を用いた癒着防止材熱架橋ゼラチンフィルムの有用性 -セルロースフィルムとの比較- (2015)
  • Author(s): 堀井常人、辻本洋行、萩原明郎 他
  • Organizer: 第14回日本再生医療学会総会
  • Place of Presentation: パシフィコ横浜(横浜市)
  • Year and Date: 2015-03-19 – 2015-03-21
• [Presentation] 再生の足場材料を用いた癒着防止材 -熱架橋gelatin filmの癌の腹膜播種性転移に与える影響について- (2015)
  • Author(s): 宮本博恵、辻本洋行、萩原明郎 他
  • Organizer: 第14回日本再生医療学会総会
  • Place of Presentation: パシフィコ横浜(横浜市)
  • Year and Date: 2015-03-19 – 2015-03-21
• [Presentation] 再生の足場材料を用いた癒着防止材 -熱架橋gelatin filmとその応用- (2014)
  • Author(s): 辻本洋行、萩原明郎 他
  • Organizer: 第22回日本消化器関連学会週間(JDDW)
  • Place of Presentation: 神戸国際会議場（神戸市）
  • Year and Date: 2014-10-23 – 2014-10-26
• [Presentation] 再生の足場材料を用いた癒着防止材 -再生医療の手法の汎用化をめざして- (2014)
  • Author(s): 辻本洋行、萩原明郎 他
  • Organizer: 第114回日本外科学会定期学術集会
  • Place of Presentation: 京都国際会議場（京都市）
  • Year and Date: 2014-04-03 – 2015-04-05
• [Presentation] 再生の足場材料を用いた癒着防止材 -再生医療の手法の汎用化をめざして- (2014)
  • Author(s): 辻本洋行、萩原明郎 他
  • Organizer: 第13回日本再生医療学会総会
  • Place of Presentation: 京都国際会館（京都市）
• [Presentation] 新規癒着防止材熱架橋gelatin filmの物理的・生物学的特性 -架橋度と癒着防止効果の関係について- (2013)
  • Author(s): 辻本洋行、萩原明郎 他
  • Organizer: 第113回日本外科学会定期学術集会
  • Place of Presentation: 福岡国際会議場（博多市）
• [Presentation] 再生の足場材料を用いた癒着防止材 -腹腔鏡手術に応用可能な新規癒着防止材gelatin flakeの特性について- (2013)
  • Author(s): 辻本洋行、萩原明郎 他
  • Organizer: 第34回日本炎症・再生医学会
  • Place of Presentation: 京都国際会館（京都市）
• [Presentation] 再生の足場材料を用いた癒着防止材 -腹腔鏡手術に応用可能な新規癒着防止材gelatin flakeの特性について- (2013)
  • Author(s): 辻本洋行、萩原明郎 他
  • Organizer: 第21回日本消化器関連学会週間(JDDW)
  • Place of Presentation: プリンスホテル高輪(東京都)