Elucidation of specific lncRNA of lymph node metastases in gastric cancer for surgical individual treatment for the gastric cancer.

• Project/Area Number: 25462018
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Digestive surgery
• Research Institution: Mie University
• Principal Investigator: Ohi Masaki 三重大学, 医学(系)研究科(研究院), 講師 (40418752)
• Co-Investigator(Kenkyū-buntansha): Kusunoki Masato 三重大学, 医学系研究科, 教授 (50192026) ; Mohri Yasuhiko 三重大学, 医学系研究科, 准教授 (70345974) ; Toiyama Yuji 三重大学, 医学系研究科, 講師 (00422824)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2014: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: lncRNAs / gastric cancer / lymph node metastases / peritoneal dissemination / lncRNA X / 胃癌 / リンパ節転移 / 遠隔転移 / lncRNA

Outline of Final Research Achievements

We have found out lncRNAs, which had high utility to be involved in metastatic formation so far to elucidate the mechanism of lymph node metastases of gastric cancer. As a result, we were able to identify lncRNA X with the strong association with lymph node metastases and peritoneal dissemination. We showed overexpression of lncRNA X in a gastric cancer tissue as compared with normal mucosa and showed that the expression of lncRNA X is the independent prognostic factor of the survival period. In addition, this lncRNA X was an independent risk factor of the peritoneal dissemination. Furthermore, as a result of having tested participation in growth of lncRNA X in vivo experiment, tumor enlargement was significantly inhibited in the gastric cell lines that were controlled by siRNA to lncRNA X.

Research Products

• [Journal Article] Circulating microRNA-203 predicts metastases, early recurrence, and poor prognosis in human gastric cancer. (2015)
  • Author(s): Imaoka H, Toiyama Y, Okigami M, Yasuda H, Saigusa S, Ohi M, Tanaka K, Inoue Y, Mohri Y, Kusunoki M.
  • Journal Title: Gastric Cancer. Volume: 印刷中
  • NAID: 40020881612
  • Peer Reviewed / Open Access
• [Journal Article] Clinical significance of RacGAP1 expression at the invasive front of gastric cancer. (2015)
  • Author(s): Saigusa S, Tanaka K, Mohri Y, Ohi M, Shimura T, Kitajima T, Kondo S, Okugawa Y, Toiyama Y, Inoue Y, Kusunoki M.
  • Journal Title: Gastric Cancer Volume: 18 Issue: 1 Pages: 84-92
  • DOI: 10.1007/s10120-014-0355-1
  • NAID: 40020580250
  • Peer Reviewed / Open Access
• [Journal Article] Clinical significance of RacGAP1 expression at the invasive front of gastric cancer. (2014)
  • Author(s): Saigusa S, Tanaka K, Mohri Y, Ohi M, Shimura T, Kitajima T, Kondo S, Okugawa Y, Toiyama Y, Inoue Y, Kusunoki M.
  • Journal Title: Ｇａｔｓｒｉｃ ｃａｎｃｅｒ Volume: -
  • NAID: 40020580250
  • Peer Reviewed
• [Journal Article] Single-incision laparoscopic and endoscopic cooperative surgery for gastrointestinal stromal tumor arising from the duodenum. (2013)
  • Author(s): Ohi M, Yasuda H, Ishino Y, Katsurahara M, Saigusa S, Tanaka K, Tanaka K, Mohri Y, Inoue Y, Uchida K, Kusunoki M.
  • Journal Title: Asian J Endosc Surg. Volume: 6 Pages: 307-310
  • Peer Reviewed
• [Presentation] 胃癌における転移および予後不良予測因子としての血清中microRNA-203発現の検討 (2015)
  • Author(s): 今岡裕基、問山裕二、浦谷亮、森浩一郎、野口智史、井出正造、沖哲、沖上正人、安田裕美、三枝晋、大井正貴、田中光司、井上靖浩、毛利靖彦、楠正人
  • Organizer: 第23回日本消化器関連学会週間
  • Place of Presentation: グランドプリンスホテル新高輪（東京都）
  • Year and Date: 2015-10-08
• [Presentation] 胃癌におけるANGPTL2の機能的、臨床病理学的意義の検証 (2014)
  • Author(s): 問山 裕二, 北嶋 貴仁, 志村 匡信, 今岡 裕基, 近藤 哲, 井出 正造, 沖上 正人, 安田 裕美, 三枝 晋, 大井 正貴, 田中 光司, 井上 靖浩, 毛利 靖彦, 楠 正人
  • Organizer: 日本癌治療学会
  • Place of Presentation: 横浜
  • Year and Date: 2014-08-28 – 2014-08-30
• [Presentation] 胃癌におけるL1CAM(L1 cell adhesion molecule)発現と臨床病理学的因子および予後との関連 (2014)
  • Author(s): 北嶋 貴仁, 田中 光司, 三枝 晋, 志村 匡信, 近藤 哲, 沖上 正人, 安田 裕美, 奥川 喜永, 問山 裕二, 大井 正貴, 井上 靖浩, 荒木 俊光, 内田 恵一, 毛利 靖彦, 楠 正人
  • Organizer: 日本外科学会
  • Place of Presentation: 京都
  • Year and Date: 2014-04-03 – 2014-04-05
• [Presentation] 食道癌に対する半腹臥位による胸腔鏡下食道切除術の工夫 (2013)
  • Author(s): 大井 正貴沖上 正人, 安田 裕美, 石野 義人, 三枝 晋, 問山 裕二, 小林 美奈子, 荒木 俊光, 井上 靖浩, 田中 光司, 毛利 靖彦, 楠 正人
  • Organizer: 日本内視鏡外科学会総会
  • Place of Presentation: 福岡
• [Presentation] 胃癌組織におけるANGPTL2タンパク発現と臨床病理学的因子及び予後に関する検討 (2013)
  • Author(s): 志村 匡信, 田中 光司, 三枝 晋, 北嶋 貴仁, 近藤 哲, 井出 正造, 今岡 裕基, 奥川 喜永, 問山 裕二, 荒木 俊光, 大井 正貴, 井上 靖浩, 内田 恵一, 毛利 靖彦, 楠 正人
  • Organizer: 日本癌治療学会総会
  • Place of Presentation: 京都