| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
|
|---|
| Outline of Final Research Achievements |
Hypoxia is substantial in solid tumors. Cancer cells under hypoxic environment are known to increase malignant characters including matastasis and drug resistance. Present study revealed that HIF-1α knockdown (KD) destroyed Warburg effect in gastric cancer cell line 58As9 under hypoxia, and induced apoptosis owing to excessive ROS production. Glucose (G) plus Insulin (I) treatment enhanced the apoptotic effect in the hypoxic 58As9 KD cells due to further ROS production. In vivo study showed that GI treatment increased apoptotic cells of 58As9 KD tumor, but not 58As9 control in nude mice, supporting in vitro data.
These results was successfully published in PLoS One paper in 2015. Thereafter, we isolated HIF-1α inhibitor YC-1 and revealed that YC-1 + GI therapy induced apoptotic effect in wild type 58As9 cells under hypoxia. At present, we are investigating whether or not YC-1 + GI therapy provides apoptotic effect on 58As9 tumor in nude mice.
|
