Analysis of the peritoneal dissemination mechanism and minimal cancer cells in gastrointestinal cancer and establishment of the anticancer agent sensitivity laboratory procedure
Project/Area Number |
25462032
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Digestive surgery
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Research Institution | Kagoshima University |
Principal Investigator |
Uenosono Yoshikazu 鹿児島大学, 医歯(薬)学総合研究科, 特任准教授 (60398279)
|
Co-Investigator(Kenkyū-buntansha) |
ARIGAMI TAKAAKI 鹿児島大学, 医歯学域医学部・歯学部附属病院, 助教 (40527058)
NATSUGOE SHOJI 鹿児島大学, 医歯学域医学系, 教授 (70237577)
柳田 茂寛 鹿児島大学, 医学部・歯学部附属病院, 特任助教 (20444903)
|
Project Period (FY) |
2013-04-01 – 2016-03-31
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Project Status |
Completed (Fiscal Year 2015)
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Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
|
Keywords | 循環癌細胞 / 腹膜播種 / 個別化化療 / NOGマウス / NOGマウス |
Outline of Final Research Achievements |
Peritoneal dissemination is one of the major metastatic patterns in patients with advanced gastric cancer. However, it is difficult to diagnose peritoneal tumor dissemination in the clinical management. The purpose of this study is to establish non -invasive diagnosis of free tumor cells in peritoneal cavity. Gastric cancer cell lines were injected into peritoneal cavity of NOG mouse, and we evaluated peritoneal washes by cytology and RT-PCR. Furthermore, peripheral blood was evaluated for detecting circulating tumor cells (CTC). In 30 advanced gastric cancer patients, we performed similar detection. CTC were found 24 hours after the dosage in mice. On the other hands, all of advanced gastric cancer patients with peritoneal dissemination and/ or cytology positive had CTCs. A non-invasive prediction of peritoneal dissemination may be possible by CTC diagnosis.
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Report
(3 results)
Research Products
(8 results)
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[Journal Article] Clinical Significance of Circulating Tumor Cells in Peripheral Blood of Patients with Esophageal Squamous Cell Carcinoma.2015
Author(s)
Matsushita D, Uenosono Y, Arigami T, Yanagita S, Nishizono Y, Hagihara T, Hirata M, Haraguchi N, Arima H, Kijima Y, Kurahara H, Maemura K, Okumura H, Ishigami S, Natsugoe S
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Journal Title
Ann Surg Oncol
Volume: 22
Issue: 11
Pages: 3674-3680
DOI
Related Report
Peer Reviewed
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[Presentation] 高度進行胃癌の化学療法における血中循環癌細胞の数とHER2発現の変化2015
Author(s)
上之園芳一, 有上貴明, 柳田茂寛, 大久保啓史, 貴島孝, 松下大輔, 有馬豪男, 奥村浩, 石神純也, 帆北修一, 夏越祥次
Organizer
第115回日本外科学会定期学術集会
Place of Presentation
名古屋国際会議場(愛知県名古屋市)
Year and Date
2015-04-18
Related Report
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[Presentation] 胃癌における血中遊離癌細胞の検出とHER2発現に基づく臨床応用への可能性
Author(s)
上之園芳一, 有上貴明, 柳田茂寛, 有馬豪男, 萩原貴彦, 原口尚士, 松下大輔, 平原徹志, 石神純也, 帆北修一, 夏越祥次
Organizer
第51回日本癌治療学会学術集会
Place of Presentation
京都市
Related Report