Analysis of novel gastric tumor suppressor RUNX3 for the clinical application of gastric cancer treatment

• Project/Area Number: 25462033
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Digestive surgery
• Research Institution: Kyoto Prefectural University of Medicine
• Principal Investigator: sakakura chouhei 京都府立医科大学, 医学(系)研究科(研究院), 特任教授 (10285257)
• Co-Investigator(Kenkyū-buntansha): OKUDA Tsukasa 京都府立医科大学, 医学研究科, 教授 (30291587) ; KODAMA Yuzou 京都大学, 医学研究科, 助教 (80373687) ; 小西 英幸 京都府立医科大学, 医学(系)研究科(研究院), 講師 (30295670) ; 児玉 裕三 京都大学, 医学(系)研究科(研究院), 助教 (80378687)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 胃癌 / 癌抑制遺伝子 / 分子生物学 / 遺伝子診断 / 発癌予防 / RUNX3

Outline of Final Research Achievements

Based on the analysis result of RUNX3 knockout mouse as well as clinical samples of gastric cancer, the following was developed aiming at the functional analysis of RUNX3, and gene diagnosis and the application to carcinogenic prevention and medical treatment.
We have already identified several kinds of markers of a gastric cancer precursor cell, and are looking for a stomach cancer precursor cell in the background membrane of an early frequent occurrence stomach cancer excision specimen. Furthermore, RUNX3 has played the role important for TGFbeta dependence apoptosis (Oncogene, Sakakura et al., 2007). The molecule (TCF4) which the carcinogenic signal transfer system (Wnt signal system) of colorectal cancer and a RUNX3-TGFbeta signal transfer system overlap is also identified (Cancer Cell, Ito et al, 2009). With Luminex system, we establish the quick serological-diagnosis system which can measure quantitatively methylation of two or more genes in many samples in a short time.

Research Products

• [Journal Article] Poor prognostic subgroup in T3N0 stage IIA gastric cancer, suggesting an indication for adjuvant chemotherapy. (2015)
  • Author(s): Imamura T, Komatsu S, Ichikawa D, Kubota T, Okamoto K, Konishi H, Shiozaki A, Fujiwara H, Morimura R, Murayama Y, Kuriu Y, Ikoma H, Nakanishi M, Sakakura C, et al.
  • Journal Title: J Surg Oncol. Volume: 111(2) Issue: 2 Pages: 221-5
  • DOI: 10.1002/jso.23796
  • Peer Reviewed
• [Journal Article] Post-hepatectomy survival in advanced hepatocellular carcinoma with portal vein tumor thrombosis. (2015)
  • Author(s): Yamamoto Y, Ikoma H, Morimura R, Shoda K, Konishi H, Murayama Y, Komatsu S, Shiozaki A, Kuriu Y, Kubota T, Nakanishi M, Ichikawa D, Fujiwara H, Okamoto K, Sakakura C, et al.
  • Journal Title: World J Gastroenterol. Volume: 21(1) Issue: 1 Pages: 246-53
  • DOI: 10.3748/wjg.v21.i1.246
  • Peer Reviewed
• [Journal Article] Evaluation of the efficacy of peritoneal lavage with distilled water in colorectal cancer surgery: in vitro and in vivo study. (2015)
  • Author(s): Takemoto K, Shiozaki A, Ichikawa D, Komatsu S, Konishi H, Nako Y, Murayama Y, Kuriu Y, Nakanishi M, Fujiwara H, Okamoto K, Sakakura C, Nakahari T, Marunaka Y, Otuji E.
  • Journal Title: J Gastroenterol. Volume: 50 Issue: 3 Pages: 287-297
  • DOI: 10.1007/s00535-014-0971-x
  • Peer Reviewed
• [Journal Article] Impact of age on early surgical outcomes of laparoscopy-assisted gastrectomy with suprapancreatic nodal dissection for clinical stage I gastric cancer. (2015)
  • Author(s): Kosuga T, Ichikawa D, Okamoto K, Komatsu S, Konishi H, Takeshita H, Shiozaki A, Fujiwara H, Morimura R, Ikoma H, Murayama Y, Kuriu Y, Nakanishi M, Sakakura C, et al.
  • Journal Title: Anticancer Res. Volume: 35(4) Pages: 2191-8
  • Peer Reviewed
• [Journal Article] Peritoneal colon cancer metastasis to bilateral inguinal hernia repair sites: report of a case. (2015)
  • Author(s): Kudou M, Murayama Y, Konishi H, Morimura R, Komatsu S, Shiozaki A, Kuriu Y, Ikoma H, Kubota T, Nakanishi M, Ichikawa D, Fujiwara H, Okamoto K, Sakakura C, et al.
  • Journal Title: Surg Today. Volume: 45(8) Issue: 8 Pages: 1053-7
  • DOI: 10.1007/s00595-014-1037-7
  • Peer Reviewed
• [Journal Article] Laparoscopic transhiatal approach for resection of an adenocarcinoma in long-segment Barrett's esophagus. (2015)
  • Author(s): Shiozaki A, Fujiwara H, Konishi H, Kinoshita O, Kosuga T, Morimura R, Murayama Y, Komatsu S, Kuriu Y, Ikoma H, Nakanishi M, Ichikawa D, Okamoto K, Sakakura C, Otsuji E.
  • Journal Title: World Journal of Gastroenterology Volume: 21 Issue: 29 Pages: 8974-8980
  • DOI: 10.3748/wjg.v21.i29.8974
  • Peer Reviewed / Open Access
• [Journal Article] Adipose-derived stem cells enhance tissue regeneration of gastrotomy closure. (2013)
  • Author(s): Komiyama S, Sakakura C, Shiozaki A, Fujiwara H, Konishi H, Morimura R, Murayama Y, Komatsu S, Kuriu Y, Ikoma H, Kubota T, Nakanishi M, Ichikawa D, Okamoto K, Otsuji E
  • Journal Title: J Surg Res Volume: 185 Pages: 945-952
  • Peer Reviewed
• [Journal Article] Positive short-term outcomes of laparoscopic sleeve gastrectomy (LSG) for the treatment of obesity and its associated comorbidities in Japanese patients. (2013)
  • Author(s): Sakakura C, Ozamoto Y, Togawa T, Hagiwara A, Eigo Otsuji E, Fujiwara H, Okamoto K, Daisuke I, Nakanishi M, Kubota T, Kuriu Y, Ikoma H, Shiozaki A, Komatsu S, Murayama Y, Morimura R, Konishi H.
  • Journal Title: J Kyoto Pref Univ Med, Volume: 33 Pages: 619-626
  • Peer Reviewed
• [Journal Article] Comparison of Prognostic Compatibility between Seventh AJCC/TNM of the Esophagus and 14th JCGC Staging Systems in Siewert Type II Adenocarcinoma. (2013)
  • Author(s): Kawaguchi T, Komatsu S, Ichikawa D, Kubota T, Okamoto K, Shiozaki A, Fujiwara H, Konishi H, Morimura R, Murayama Y, Kuriu Y, Ikoma H, Nakanishi M, Sakakura C, Otsuji E.
  • Journal Title: Anticancer Res, Volume: 33 Pages: 3461-3465
  • Peer Reviewed
• [Journal Article] Novel technique for dissection of subcarinal and main bronchial lymph nodes using a laparoscopic transhiatal approach for esophageal cancer. (2013)
  • Author(s): Shiozaki A, Fujiwara H, Konishi H, Morimura R, Murayama Y, Komatsu S, Kuriu Y, Ikoma H, Kubota T, Nakanishi M, Ichikawa D, Okamoto K, Sakakura C, Otsuji E.
  • Journal Title: Anticancer Res Volume: 33 Pages: 2577-2585
  • Peer Reviewed
• [Journal Article] COX-2 overexpression Induced by gene transfer reduces sensitivity of TE13 esophageal carcinoma cells to 5-fluorouracil and cisplatin. (2013)
  • Author(s): Okamura H, Fujiwara H, Umehara S, Okamura S, Todo M, Furutani A, Yoneda M, Shiozaki A, Komatsu S, Kubota T, Ichikawa D, Okamoto K, Ochiai T, Sakakura C, Takahashi Y, Yoshimoto T, Otsuji E.
  • Journal Title: Anticancer Res, Volume: 33 Pages: 537-542