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Functional analysis of GCF2 in tumor microenvironment

Research Project

Project/Area Number 25462045
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Digestive surgery
Research InstitutionTohoku University

Principal Investigator

Ohtsuka Hideo  東北大学, 大学病院, 助教 (50451563)

Co-Investigator(Kenkyū-buntansha) Motoi Fuyuhiko  東北大学, 医学系研究科, 准教授 (30343057)
Fukase Koji  東北大学, 大学病院, 助教 (00578677)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
KeywordsGCF2 / LRRFIP1 / EMT / Wnt signal / 膵癌 / β-catenin / Wnt / 上皮間葉移行 / Wntシグナル
Outline of Final Research Achievements

The canonical Wnt/β-catenin signaling pathway has been shown to promote the epithelial-mesenchymal transition (EMT), which is a crucial process in multiple embryonic developmental processes and the progression of carcinomas. In the present study, we identified the signaling elements targeted by GCF2 for promotion of the EMT in pancreatic cancer. GCF2 silencing reversed the EMT, as shown by increased expression of E-cadherin (an epithelial marker) and decreased expression of vimentin (a mesenchymal marker). Silencing of GCF2 up-regulated phosphorylation of β-catenin and decreased its nuclear localization by targeting β-catenin destruction complex. The migration and invasion capabilities were strongly inhibited in GCF2 knocked down pancreatic cancer cells. Consequently, our data strongly suggested that LRRFIP1 played an important role in the invasion of carcinoma cells and as an attractive candidate for targeted therapy in human cancers.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • Research Products

    (3 results)

All 2015 2013

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Acknowledgement Compliant: 1 results) Presentation (2 results)

  • [Journal Article] Silencing of LRRFIP1 reverses the epithelial-mesenchymal transition via inhibition of the Wnt/β-catenin signaling pathway.2015

    • Author(s)
      Douchi D, Ohtsuka H, Ariake K, Masuda K, Kawasaki S, Kawaguchi K, Fukase K, Oikawa M, Motoi F, Naitoh T, Katayose Y, Egawa S, Unno M.
    • Journal Title

      Cancer Letters

      Volume: 365 (1) Issue: 1 Pages: 132-140

    • DOI

      10.1016/j.canlet.2015.05.023

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] Functional analysis of GCF2/LRRFIP1 in the tumor microenvironment2013

    • Author(s)
      堂地大輔、大塚英郎、海野倫明
    • Organizer
      第22回日本がん転移学会学術集会
    • Place of Presentation
      松本
    • Related Report
      2013 Research-status Report
  • [Presentation] がん微小環境における新規細胞骨格調節因子GCF2/LRRFIP1の機能解析2013

    • Author(s)
      堂地大輔, 大塚英郎, 有明恭平, 深瀬耕二, 吉田 寛, 元井冬彦, 内藤 剛, 片寄 友, 江川新一, 海野倫明
    • Organizer
      第68回日本消化器外科学会総会
    • Place of Presentation
      宮崎
    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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