In vivo imaging system of dynamics of molecularly targeted drugs on the metastatic tumor xenografts using a multiphoton microscopy
Project/Area Number |
25462052
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Digestive surgery
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Research Institution | Mie University |
Principal Investigator |
TANAKA KOJI 三重大学, 医学部附属病院, 講師 (10345986)
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Co-Investigator(Kenkyū-buntansha) |
Toiyama Yuji 三重大学, 医学系研究科, 助教 (00422824)
Inoue Yasuhiro 三重大学, 医学部附属病院, 講師 (20324535)
Uchida Keiichi 三重大学, 医学部附属病院, 准教授 (30293781)
Mohri Yasuhiko 三重大学, 医学系研究科, 准教授 (70345974)
Mizoguchi Akira 三重大学, 医学系研究科, 教授 (90181916)
Kusunoki Masato 三重大学, 医学系研究科, 教授 (50192026)
|
Project Period (FY) |
2013-04-01 – 2016-03-31
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Project Status |
Completed (Fiscal Year 2015)
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Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥260,000 (Direct Cost: ¥200,000、Indirect Cost: ¥60,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2013: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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Keywords | 二光子励起顕微鏡 / 癌転移 / 生体内リアルタイムイメージング / 癌微小環境 / 抗体医薬 / 生体内イメージング / 癌転移巣 / 分子標的薬 |
Outline of Final Research Achievements |
We developed a method of in vivo optical pathology using a multiphoton microscopy (MPM) on metastatic tumor xenograft model (colorectal liver metastasis and gastric peritoneal metastasis), and evaluated chemotherapeutics efficacy in the tumor microenvironment. We imaged the binding of Alexa Fluor labeling anti-epidermal growth factor receptor (EGFR) antibody (cetuximab) to EGFR on viable HT29 cells (colorectal cancer cell line) in vitro without cell fixation. Similarly, we imaged the binding of Alexa Fluor labeling anti- c-MET (hepatocyte growth factor receptor) antibody to MET on viable NUGC4 cells (gastric cancer cell line). We imaged the binding of fluorescently labeled antibodies on tumor cells via their antigens on the metastatic tumor xenografts using a MPM in vivo real-time. In vivo imaging system of dynamics of such drugs.on the metastatic tumor xenografts will be useful to clarify in vivo pharmacokinetic and pharmacodynamic mechanisms.
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Report
(3 results)
Research Products
(5 results)
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[Journal Article] Visualization of in vivo thromboprophylactic and thrombolytic efficacy of enoxaparin in laser-induced vascular endothelial injury model using multiphoton microscopy.2015
Author(s)
Tanaka K, Koike Y, Matsushita K, Okigami M, Toiyama Y, Kawamura M, Saigusa S, Okugawa Y, Inoue Y, Uchida K, Araki T, Mohri Y, Mizoguchi A, Kusunoki M.
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Journal Title
Am J Transl Res.
Volume: 7
Pages: 79-87
Related Report
Peer Reviewed
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[Journal Article] In vivo optical pathology of paclitaxel efficacy on the peritoneal metastatic xenograft model of gastric cancer using two-photon laser scanning microscopy2014
Author(s)
Shimura T, Tanaka K, Toiyama Y, Okigami M, Ide S, Kitajima T, Kondo S, Saigusa S, Ohi M, Araki T, Inoue Y, Uchida K, Mohri Y, Mizoguchi A, Kusunoki M
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Journal Title
Gastric Cancer
Volume: (in press)
Issue: 1
Pages: 109-118
DOI
Related Report
Peer Reviewed
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[Journal Article] In vivo optical pathology of paclitaxel efficacy on the peritoneal metastatic xenograft model of gastric cancer using two-photon laser scanning microscopy.2014
Author(s)
Shimura T, Tanaka K, Toiyama Y, Okigami M, Ide S, Kitajima T, Kondo S, Saigusa S, Ohi M, Araki T, Inoue Y, Uchida K, Mohri Y, Mizoguchi A, Kusunoki M.
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Journal Title
Gastric Cancer.
Volume: Jan 8
NAID
Related Report
Peer Reviewed
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[Presentation] Development of in vivo imaging system of molecularly targeted drugs using multiphoton microscopy2013
Author(s)
Koji Tanaka, Tadanobu Shimura, Masato Okigami, Yuji Toiyama, Yoshinaga Okugawa, Takahito Kitajima, Satoru Kondo, Junichiro Hiro, Yasuhiro Inoue, Yasuhiko Mohri, Minako Kobayashi, Chikao Miki, and Masato Kusunoki
Organizer
The 72nd Annual Meeting of the Japanese Cancer Association
Place of Presentation
PACIFICO Yokohama
Related Report
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