Prediction of lateral lymph node metastasis by inspection of the para-rectal lymph nodes using the one-step nucleic acid amplification (OSNA) assay in the lower rectal cancer

• Project/Area Number: 25462055
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Digestive surgery
• Research Institution: Osaka University
• Principal Investigator: Hata Taishi 大阪大学, 医学(系)研究科(研究院), 助教 (70644912)
• Co-Investigator(Kenkyū-buntansha): YAMAMOTO Hirofumi 大阪大学, 大学院医学系研究科, 教授 (30322184) ; 竹山 廣志 近畿大学, 医学部附属病院, 講師 (60621018)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 直腸癌 / OSNA法 / 側方郭清 / 術中迅速検査 / 術中迅速診断

Outline of Final Research Achievements

We have investigated 26 cases with lower rectal cancer who received lateral lymph node dissection. Twenty-three cases were treated with pre-operative chemotherapy. The diagnostic performance of OSNA assay of para-rectal lymph nodes to the lateral lymph node metastasis was as follows.; Correct diagnosis 85%, Sensitivity 80%, Specificity 86%, Negative predictive value 95%. Although most cases had preoperative chemotherapy, negative prediction of the lateral lymph node metastasis was still highly achieved by OSNA assay (95%) when targeted to para-rectal lymph nodes. The present study suggests a possibility that intra-operative evaluation of para-rectal lymph nodes by OSNA assay may be useful to judge the necessity of lateral lymph node dissection. A large scale conformation study would be essential.