Development of cancer treatment harnessing NKT cells with a cross-talk of innate and acquired immunity

• Project/Area Number: 25462056
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Digestive surgery
• Research Institution: Kobe University
• Principal Investigator: Yamashita Kimihiro 神戸大学, 医学部附属病院, 特命助教 (80535427)
• Co-Investigator(Kenkyū-buntansha): SUMI Yasuo 神戸大学, 大学院医学研究科, 助教 (00529521) ; NAKAMURA Tetsu 神戸大学, 医学部附属病院, 助教 (10403247) ; KAMIGAKI Takashi 神戸大学, 大学院医学研究科, 客員教授 (20372641) ; KAKEJI Yoshihiro 神戸大学, 大学院医学研究科, 教授 (80284488)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 腫瘍免疫 / NKT細胞 / 大腸癌

Outline of Final Research Achievements

The aim of this study is show allogeneic DCG will work stronger than syngeneic DCG in terms of anti-tumor effect in mouse lung metastasis treatment model of NKT cells that act on the innate and acquired immunity system. By introducing tumor antigen into allogeneic or syngeneic DCG, we will elucidate to generate tumor antigen-specific T cells in this system, and moreover, we will create the system to analyze the functions and sequential differentiation of them.

Research Products

• [Journal Article] Early-phase Treatment by Low-dose 5-Fluorouracil or Primary Tumor Resection Inhibits MDSC-mediated Lung Metastasis Formation. (2015)
  • Author(s): Otsubo D, Yamashita K, Fujita M, Nishi M, Kimura Y, Hasegawa H, Suzuki S, Kakeji Y
  • Journal Title: Anticancer Res. Volume: 35 Pages: 4425-4431
• [Journal Article] 癌の遺伝子診断・治療（総説） (2015)
  • Author(s): 山下 公大、西 将康、掛地 吉弘
  • Journal Title: 消化器外科学レビュー2015-'16 Volume: - Pages: 186-190
• [Journal Article] Immunotherapy of autologous tumor lysate-loaded dendritic cell vaccines by a closed-flow electroporation system for solid tumors. (2013)
  • Author(s): Kamigaki T, Kaneko T, Naitoh K, Takahara M, Kondo T, Ibe H, Matsuda E, Maekawa R, Goto S.
  • Journal Title: Anticancer Res. Volume: 33(7) Pages: 2971-2976
  • Peer Reviewed
• [Presentation] NKT細胞活性化の抗腫瘍効果におけるアロ樹状細胞の有用性 (2015)
  • Author(s): 山下 公大、西 将康、田中 智子、山本 将士、金治 新悟、金光 聖哲、角 泰雄、中村 哲、鈴木 知志、掛地 吉弘
  • Organizer: 第24回日本がん転移学会学術集会・総会
  • Place of Presentation: 大阪
  • Year and Date: 2015-07-23
• [Presentation] Early-phase administration of low-dose 5-fluorouracil and early-phase resection of the established melanoma prevents the formation of lung metastases by inhibiting MDSC generation and accumulation (2015)
  • Author(s): Yamashita K, Fujita M, Nishi M, Otsubo D, Suzuki S, Kakeji Y.
  • Organizer: Interｎational Conference of Cancer Immunotherapy and Macrophages 2015
  • Place of Presentation: 東京
  • Year and Date: 2015-07-09
  • Int'l Joint Research
• [Presentation] 肺転移における骨髄由来抑制性細胞の役割 (2013)
  • Author(s): 山下 公大、大坪 大、長谷川 寛、裏川 直樹、鈴木 知志、田中 賢一、掛地 吉弘
  • Organizer: 第22回 日本癌病態治療研究会
  • Place of Presentation: 東京