The analysis of the spindle checkpoint protens and the application to the new molecular targetted therapy for colorectal cancer.

• Project/Area Number: 25462059
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Digestive surgery
• Research Institution: Kyushu University
• Principal Investigator: Emi Yasunori 九州大学, 医学(系)研究科(研究院), 研究員 (50223673)
• Co-Investigator(Kenkyū-buntansha): MORITA Masaru 独立行政法人国立病院機構(九州がんセンター臨床研究センター), その他部局等, その他 (30294937) ; KITAO Hiroyuki 九州大学, 医学研究院, 准教授 (30368617) ; OKI Eiji 九州大学, 大学病院, 講師 (70380392) ; SAEKI Hiroshi 九州大学, 医学研究院, 准教授 (80325448) ; 安藤 幸滋 九州大学, 大学病院, 助教 (20608864)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 大腸癌 / ゲノム不安定性 / 細胞分裂期スピンドルチェックポイント機構 / p53 / MSI / BUBR1

Outline of Final Research Achievements

To elucidate the clinical significance of spindle checkpoint protein in colorectal cancer, and to find the new candidate for molecular targeted therapy, we analyzed the expression of the spindle checkpoint proteins in colorectal clinical samples. We investigated the interplay between BUBR1 and p53 and their association with genetic instability in colorectal cancer. In 139 colorectal cases, cases with high BUBR1 expression and TP53 mutation had profound aneuploidy phenotypes and less frequent MSI. On the other hand, high expression of PLK1 was also associated with DNA aneuploidy in clinical gastric cancer specimens. High expression of PLK1 could have detrimental effects in tumors with DNA aneuploidy. These findings indicate that spindle checkpoint proteins might contribute cooperatively to the DNA aneuploidy and the carcinogenesis.

Research Products

• [Journal Article] Initial report of KSCC0803: feasibility study of capecitabine as adjuvant chemotherapy for stage III colon cancer in Japanese patients. (2013)
  • Author(s): Emi Y., Kakeji Y., Oki E., Saeki H., Ando K.,
  • Journal Title: Int J Clin Oncol Volume: 18 Pages: 254-259
  • NAID: 10031176263
  • Peer Reviewed
• [Journal Article] Newly Developed Liver-Retraction Method for Laparoscopic Gastric Surgery Using a Silicone Disc: the Φ-Shaped Technique. (2013)
  • Author(s): "Saeki H., Oki E., Kawano H., Ando K., Ida S.,
  • Journal Title: Journal of the American College of Surgeons Volume: 216
  • Peer Reviewed
• [Journal Article] Totally Laparoscopic Distal Gastrectomy for Elderly Patients with Gastric Cancer. (2013)
  • Author(s): Oki E., Sakaguchi Y., Ohgaki K., Saeki H., Ikegami T., Minami K., Yamashita Y., Toh Y., Soejima Y., Ando K., Mimori K., Watanabe M., Sugimachi K., Uchiyama H., Yoshizumi T., Kawanaka H., Morita M., Ikeda T., Maehara Y.
  • Journal Title: Fukuoka Acta Medica Volume: 104 Pages: 290-298
  • NAID: 120005372094
  • Peer Reviewed
• [Journal Article] Mortalin is a prognostic factor of gastric cancer with normal p53 function. (2013)
  • Author(s): Ando K., Oki E., Zhao Y., Yoshida A., Kitao H., Saeki H., Kimura Y., Ida S., Morita M., Kusumoto T., Maehara Y.
  • Journal Title: Gastric Cancer Volume: 17 Pages: 255-262
  • NAID: 40020216055
  • Peer Reviewed
• [Journal Article] Advances in esophageal surgery in elderly patients with thoracic esophageal cancer. (2013)
  • Author(s): "Morita M., Kawano H., Otsu H., Kimura K., Saeki H.,
  • Journal Title: Anticancer Res Volume: 33 Pages: 1641-1647
  • Peer Reviewed
• [Journal Article] Surgical resection for esophageal cancer synchronously or metachronously associated with head and neck cancer. (2013)
  • Author(s): "Morita M., Kawano H., Otsu H., Kimura K., Saeki H.,
  • Journal Title: Ann Surg Oncol Volume: 20 Pages: 2434-2439
  • Peer Reviewed
• [Journal Article] Esophageal replacement by colon interposition with microvascul.ar surgery for patients with thoracic esophageal cancer -the utility of superdrainage. (2013)
  • Author(s): Saeki H., Morita M., Harada N., Egashira A., Oki E., Uchiyama H., OhgaT., Kakeji Y., Sakaguchi Y., Maehara Y.
  • Journal Title: Diseases of the Esophagus Volume: 26 Pages: 50-56
  • Peer Reviewed
• [Journal Article] Multimodal treatment strategy for clinical T3 thoracic esophageal cancer. (2013)
  • Author(s): "Saeki H., Morita M., Tsuda Y., Hidaka G., Kasagi Y.,
  • Journal Title: Annals of Surgical Oncology Volume: 20 Pages: 4267-4273
  • Peer Reviewed
• [Presentation] Analysis of KRAS/NRAS, PI3CA and BRAF mutations in the phase 2 KSCC0901 study of cetuximab + s-1 as 3rd-line treatment for metastatic colorectal cancer in Japanese patients (2014)
  • Author(s): Yasunori Emi, Eiji Oki, Hiroshi Saeki, Hiroyuki Kitao, Hironori Samura, Takao Takahashi, Terumitsu Sawai, Yoshito Akagi, Akihito Tsuji, Susumu Eguchi, Kazuhiro Yoshida, Hideo Baba, Yutaka Ogata, Shoji Natsugoe, Mototugu Shimokawa, Yoshihiko Maehara
  • Organizer: ASCO-GI
  • Place of Presentation: San Francisco, USA
• [Presentation] Significance and accurate of HER2 evaluation as a new biomarker in advanced gastric cancer. (2013)
  • Author(s): Kimura Y., Oki E., Otsu H., Tsuda Y., Kasagi Y., Hidaka G., Zaitsu Y., Yukaya T., Kawano H., Ando K., Ida S., Aishima S., Saeki H., Morita M., Kusumoto T., Maehara Y.
  • Organizer: The 10th International Gastric Cancer Congress(IGCC 2013)
  • Place of Presentation: Verona, Italy
• [Presentation] Phase II study of docetaxel (DTX) and s-1 as neoadjuvant chemotherapy for potentially R0 advanced gastric cancer (2013)
  • Author(s): Yasunori Emi, Tetsuya Kusumoto, Hiroshi Matsuura, Mototsugu Shimokawa, Yoshihiro Kakeji, Hideo Baba, Yoshihiko Maehara
  • Organizer: The 10th International Gastric Cancer Congress(IGCC 2013)
  • Place of Presentation: Verona, Italy
• [Remarks] 九州大学大学院 消化器・総合外科
  • URL: http://www.kyu2geka.com/