The explore of new cancer stem cell marker based on intracellular behavior of biological defense factor

• Project/Area Number: 25462061
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Digestive surgery
• Research Institution: Nagasaki University
• Principal Investigator: GOTO Shinji 長崎大学, 原爆後障害医療研究所, 助教 (50186889)
• Co-Investigator(Kenkyū-buntansha): LI Taosheng 長崎大学, 原爆後障害医療研究所, 教授 (50379997) ; KAWAKATSU Miho 長崎大学, 病院(医学系), 客員研究員 (20398150)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: がん幹細胞 / がん幹細胞マーカー / 抗がん剤耐性 / 栄養飢餓耐性 / グルタチオンS-トランスフェラーゼ / GSTπ / 核局在 / 大腸がん

Outline of Final Research Achievements

We isolated candidate of cancer stem cell which has resistance to nutrient starvation and anticancer drugs such as doxorubicin and cisplatin from HCT8 human colon cancer cell line by evaluation of intracellular localization of Glutathione S-transferase pi.
We have been investigating cell surface molecules by global analysis to explore new cancer stem cell marker.

Research Products

• [Journal Article] Enhanced autophagy in colorectal cancer stem cells does not contribute to radio-resistance (2016)
  • Author(s): Chen Yan, Lan Luo, Shinji Goto, Yoshishige Urata, Chang-Ying Guo, Hanako Doi, Kaio Kitazato, Tao-Sheng Li
  • Journal Title: Oncotarget Volume: 印刷中 Issue: 29 Pages: 45112-45121
  • DOI: 10.18632/oncotarget.8972
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Nicaraven attenuates radiation-induced injury in hematopoietic stem/progenitor cells in mice (2013)
  • Author(s): Kawakatsu M, Urata Y, Imai R, Goto S, Ono Y, Nishida N, Li TS
  • Journal Title: PLoS One Volume: 8 Issue: 3 Pages: e60023-e60023
  • DOI: 10.1371/journal.pone.0060023
  • NAID: 120006985963
  • Peer Reviewed
• [Journal Article] Placental extract protects bone marrow-derived stem/progenitor cells against radiation injury through anti-inflammatory activity (2012)
  • Author(s): Kawakatsu et al
  • Journal Title: J Radiat Res Volume: 54(2) Issue: 2 Pages: 268-76
  • DOI: 10.1093/jrr/rrs105
  • Peer Reviewed
• [Presentation] The mechanism of drug resistance of CD133-expressed cancer cell (2015)
  • Author(s): 後藤信治、浦田芳重、小野悠介、李桃生
  • Organizer: 日本生化学会
  • Place of Presentation: 神戸国際会議場(神戸市、兵庫県）
  • Year and Date: 2015-12-01