Assessment of molecular marker for diagnosis of extramural tumor deposit and distant metastasis

• Project/Area Number: 25462073
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Digestive surgery
• Research Institution: Hyogo Medical University
• Principal Investigator: Masafumi Noda 兵庫医科大学, 医学部, 講師 (30309463)
• Co-Investigator(Kenkyū-buntansha): TOMITA Naohiro 兵庫医科大学, 医学部, 教授 (00252643) ; 山野 智基 兵庫医科大学, 医学部, 講師 (00599318)
• Co-Investigator(Renkei-kenkyūsha): YAMANO Tomoki 兵庫医科大学, 医学部, 講師 (00599318)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: リンパ節外転移 / 大腸癌 / 分子マーカー / CD82 / CD274

Outline of Final Research Achievements

Extramural tumor deposits (TDs) have been considered as prognostic factor for colorectal cancer. Here we tried to establish the diagnosis of TDs, which has been under discussion for years.
1.We classified EX into two type (invasive-type and nodular-type). Then we reviewed 385 patients with T3-4, N0-2, M0 who received curative resection by TDs and extracapsular lymph node involvement. iTD was a significant independent adverse prognostic factor for DSS in rectal cancer, metastasis to the liver in colorectal cancer, and distant LN metastasis in colon cancer. ECLNI was a significant independent prognostic factor for RFS in colon cancer. (BMC cancer 2015 15:859)
2. We compared the gene expression of the patients with TDs alone and those with TDs and distant metastasis by microarray. The pathway analysis showed p53 pathway has been most influenced. Gene expressions of CD82, CD274, DACH1, CXCL3, and FGF9 were significantly different between the two groups.

Research Products

• [Journal Article] Prognostic significance of classified extramural tumor deposits and extracapsular lymph node invasion in T3–4 colorectal cancer: a retrospective singlecenter study (2015)
  • Author(s): Tomoki Yamano, Shuho Semba, Masafumi Noda, Mie Yoshimura, Masayoshi Kobayashi, Michiko Hamanaka, Naohito Beppu, Aya Yano, Kiyoshi Tsukamoto, Nagahide Matsubara, Naohiro Tomita
  • Journal Title: BMC Cancer Volume: 15 Issue: 1 Pages: 1-9
  • DOI: 10.1186/s12885-015-1885-6
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] 大腸癌においてリンパ節外転移・遠隔転移に関与する遺伝子の検索 (2015)
  • Author(s): 山野智基、浜中美千子、馬場谷彰仁、吉村美衣、塚本潔、野田雅史、松原長秀、冨田尚裕
  • Organizer: 第70回 日本大腸肛門病学会
  • Place of Presentation: 名古屋観光ホテル・ヒルトン名古屋（愛知県・名古屋市）
  • Year and Date: 2015-11-19
• [Presentation] 浸潤型リンパ節外転移はT3-4、N0-2大腸癌の予後因子である (2015)
  • Author(s): 山野智基、仙波秀峰、野田雅史 、吉村美衣 、小林政義、濱中美千子、別府直仁 、矢野綾 、塚本潔、松原長秀、冨田尚裕
  • Organizer: 第53回 日本癌治療学会
  • Place of Presentation: 京都国際会館（京都府・京都市）
  • Year and Date: 2015-10-29
• [Presentation] 大腸癌治癒切除症例におけるリンパ節外転移、リンパ節外進展の検討 (2015)
  • Author(s): 山野智基、仙波秀峰、小林政義 、吉村美衣 、別府直仁、 濱中美千子、塚本潔、野田雅史 、松原長秀、冨田尚裕
  • Organizer: 第115回 日本外科学会
  • Place of Presentation: 名古屋国際会議場（愛知県・名古屋市）
  • Year and Date: 2015-04-16
• [Presentation] リンパ節外転移・浸潤陽性は大腸癌において再発高危険因子である (2013)
  • Author(s): 山野智基、野田雅史、 塚本潔、山岸大介、浜中美衣、別府直仁 小林政義、松原長秀 、冨田尚裕
  • Organizer: 第51回日本癌治療学会学術集会
  • Place of Presentation: 京都