| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Outline of Final Research Achievements |
We previously reported that the ATP-sensitive potassium (K-ATP) channel existed in mitochondria of rat hepatocyte, and K-ATP channel opener (diazoxide: Dz) promoted regeneration of remnant liver after hepatectomy. Furthermore, we showed that diazoxide decreased Ca ion release from intracellular Ca ion storing organellae in isolated hepatocytes. To solve the reason why K-ATP channel opener enhances liver regeneration, we analysed gene expression cyclopaedically. As a result, mRNAs of RyR2 and calmodulin decreased in Dz group with a significant difference. In related genes to apoptosis, the expression of caspase-3 and BAX changed. DNA microarray analysis showed the upregulations of genes related to cell adhesion and neural factor.
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