Promoting liver regeneration using an ATP-sensitive K+ channel opener
Project/Area Number |
25462077
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Digestive surgery
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Research Institution | Akita University |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
山本 雄造 秋田大学, 医学(系)研究科(研究院), 教授 (70281730)
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Project Period (FY) |
2013-04-01 – 2017-03-31
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Project Status |
Completed (Fiscal Year 2016)
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Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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Keywords | K-ATPチャネル / 肝細胞 / 残肝増殖能 / Diazoxide / ミトコンドリア / マイクロアレイ / カルシウムチャネル / mRNA / カルシウムイオン / イオンチャネル / K-ATPチャネル |
Outline of Final Research Achievements |
We previously reported that the ATP-sensitive potassium (K-ATP) channel existed in mitochondria of rat hepatocyte, and K-ATP channel opener (diazoxide: Dz) promoted regeneration of remnant liver after hepatectomy. Furthermore, we showed that diazoxide decreased Ca ion release from intracellular Ca ion storing organellae in isolated hepatocytes. To solve the reason why K-ATP channel opener enhances liver regeneration, we analysed gene expression cyclopaedically. As a result, mRNAs of RyR2 and calmodulin decreased in Dz group with a significant difference. In related genes to apoptosis, the expression of caspase-3 and BAX changed. DNA microarray analysis showed the upregulations of genes related to cell adhesion and neural factor.
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Report
(4 results)
Research Products
(19 results)
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[Journal Article] The Transformation of a Nonfunctioning Islet Cell Tumor of the Pancreas into a Proinsulinoma under Conditions of Lung Metastasis2015
Author(s)
Yoshioka M, Shibata S, Uchinami H, Watanabe G, Miyazawa H, Iida M, Yoshida M, Yoshioka T, Nanjo H, Yamamoto Y.
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Journal Title
Internal Medicine
Volume: 54
Issue: 7
Pages: 785-790
DOI
NAID
ISSN
0918-2918, 1349-7235
Related Report
Peer Reviewed / Open Access
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