Role of CLEC-2 in hepatic regeneration and fibrosis
| Project/Area Number |
25462083
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | University of Yamanashi |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
KONO Hiroshi 山梨大学, 総合研究部, 講師 (40322127)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 肝類洞壁細胞 / 肝再生 / 肝障害 / マクロファージ / 肝腫瘍 / CLEC-2 / 血小板 / 肝類洞壁内皮細胞 / 肝切除後肝再生 |
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| Outline of Final Research Achievements |
The aim of the present study was to investigate the role of CLEC-2 in liver regeneration following partial liver resection in mice. Irradiated chimeric mice transplanted with the fetal liver cells from wild-type (WT) or CLEC-2 deleted specifically from platelets (flKO) mice were generated. Mice underwent 70% of partial hepatectomy (PH). Liver/body weight ratio and the expression of all cell proliferation markers were significantly lower in the flKO group than in the WT group. The expression of pSTAT3 was significantly greater in the WT group than in the flKO group. The expression of IL-6 was significantly greater in the WT group than in the flKO group. The expression of podoplanin was detected in the hepatic sinusoids of both two groups. The extent to which platelets accumulated in hepatic sinusoids was significantly less in the flKO group than in the WT group. CLEC-2 was involved in hepatic regeneration after liver resection.
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Report
(3 results)
Research Products
(4 results)
