Role of CLEC-2 in hepatic regeneration and fibrosis

• Project/Area Number: 25462083
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Digestive surgery
• Research Institution: University of Yamanashi
• Principal Investigator: AMEMIYA Hidetake 山梨大学, 総合研究部, 助教 (70377547)
• Co-Investigator(Kenkyū-buntansha): KONO Hiroshi 山梨大学, 総合研究部, 講師 (40322127)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 肝類洞壁細胞 / 肝再生 / 肝障害 / マクロファージ / 肝腫瘍 / CLEC-2 / 血小板 / 肝類洞壁内皮細胞 / 肝切除後肝再生

Outline of Final Research Achievements

The aim of the present study was to investigate the role of CLEC-2 in liver regeneration following partial liver resection in mice. Irradiated chimeric mice transplanted with the fetal liver cells from wild-type (WT) or CLEC-2 deleted specifically from platelets (flKO) mice were generated. Mice underwent 70% of partial hepatectomy (PH). Liver/body weight ratio and the expression of all cell proliferation markers were significantly lower in the flKO group than in the WT group. The expression of pSTAT3 was significantly greater in the WT group than in the flKO group. The expression of IL-6 was significantly greater in the WT group than in the flKO group. The expression of podoplanin was detected in the hepatic sinusoids of both two groups. The extent to which platelets accumulated in hepatic sinusoids was significantly less in the flKO group than in the WT group. CLEC-2 was involved in hepatic regeneration after liver resection.

Research Products

• [Presentation] 新規クラス血小板上受容体c-type lectin-like receptor 2 (CLEC-2)は、類洞内皮との相互作用で肝切除後肝再生の早期に重要な役割を果たす (2015)
  • Author(s): 河野 寛、古屋信二、原倫生、平山和義、藤井秀樹
  • Organizer: 第36回日本炎症･再生医学会 2015年7月21-22日
  • Place of Presentation: 六本木ヒルズ 東京都
  • Year and Date: 2015-07-21
• [Presentation] 肝切除後肝再生における新規クラス血小板上受容体c-type lectin-like receptor 2 (CLEC-2)の役割 (2015)
  • Author(s): 河野 寛、古屋信二、原倫生、土屋雅人、藤井秀樹
  • Organizer: 第51回日本肝臓学会総会 2015年5月21日－22日
  • Place of Presentation: ホテル日航熊本 熊本県熊本市
  • Year and Date: 2015-05-21
• [Presentation] 肝切除後肝再生における新規クラス血小板上受容体c-type lectin-like receptor 2 (CLEC-2)の役割 (2014)
  • Author(s): 河野 寛，藤井秀樹，原 倫生，古屋信二，平山和義
  • Organizer: 第50回日本腹部救急医学会総会
  • Place of Presentation: 京王プラザホテル（東京都新宿区）
• [Presentation] 肝切除後肝再生における新規クラス血小板上受容体c-type lectin-like receptor 2 (CLEC-2)の役割 (2013)
  • Author(s): 河野 寛，藤井秀樹，原 倫生，古屋信二，平山和義
  • Organizer: 第113回日本外科学会定期学術集会
  • Place of Presentation: 福岡国際会議場（福岡県福岡市）