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Development of the innovative hybrid artificial liver with establishment of the hepatocyte strain from human iPS cells

Research Project

Project/Area Number 25462093
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Digestive surgery
Research InstitutionNational Hospital Organization, Kyushu Cancer Center (2015)
Kyushu University (2013)

Principal Investigator

YAMASHITA YOICHI  独立行政法人国立病院機構(九州がんセンター臨床研究センター), その他部局等, その他 (00404070)

Co-Investigator(Kenkyū-buntansha) Yonemitsu Yoshikazu  九州大学, 薬学研究院, 教授 (40315065)
Ikeda Tetsuo  九州大学, 医学研究院, 准教授 (60585701)
Shirabe Ken  九州大学, 医学研究院, 准教授 (70264025)
Maehara Yoshihiko  九州大学, 医学研究院, 教授 (80165662)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
KeywordsヒトiPS細胞 / ハイブリッド型人工肝臓 / 人工肝臓
Outline of Final Research Achievements

We newly established the well-differentiated liver cell line which has ammonia metabolism from human iPS cells. The metabolic detoxification ability is one of the highest specific liver functions, therefore, this cell line is thought to be the leading cell source at the time of clinical application of the artificial liver support system.
The ultimate hybrid artificial liver should be a “dropper type” rather than an extracorporeal type, therefore, we used the decellularized liver as a scaffold. Because of the vulnerability of the outer wall of the decellularized liver, the reinforcement using the “polyglycolic acid sheet + fibrin/thrombin solution” was performed. We succeeded the extracorporeal circulation for approximately one hour of rat decellularized liver. Using this experimental system, we evaluated the functions of the rat decellularized liver implanted with rat primary hepatocytes, and confirmed the high rate of ammonia metabolism.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • Research Products

    (6 results)

All 2015 2013

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (4 results) (of which Int'l Joint Research: 1 results,  Invited: 2 results)

  • [Journal Article] Surgical results for recurrent hepatocellular carcinoma after curative hepatectomy: Repeat hepatectomy vs. salvage living donor livertransplantation.2015

    • Author(s)
      Yamashita Y, Yoshida Y, Kurihara T, Itoh S, Harimoto N, Ikegami T, Yoshizumi T, Uchiyama H, Shirabe K, Maehara Y.
    • Journal Title

      Liver Transpl.

      Volume: 21 Pages: 961-8

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Surgical Outcomes of Hepatic Resection for Hepatitis B Virus Surface Antigen-Negative and Hepatitis C Virus Antibody-Negative Hepatocellular Carcinoma.2015

    • Author(s)
      Yamashita YI, Imai D, Bekki Y, Kimura K, Matsumoto Y, Nakagawara H, kegami T, Yoshizumi T, Shirabe K, Aishima S, Maehara Y
    • Journal Title

      Ann Surgical Oncol

      Volume: 22 Pages: 2279-85

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Presentation] Fundamental technology for the creation of Whole Liver Engineering, and2015

    • Author(s)
      Yo-ichi Yamashita他7名
    • Organizer
      2015 4th Tissue Engineering and Regenerative Medicine International Society World Congress
    • Place of Presentation
      Boston Marriott Copley Place Boston USA
    • Year and Date
      2015-09-08
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 医工連携による実用的肝グラフト構築を目指して2015

    • Author(s)
      山下洋市他7名
    • Organizer
      第51回九大生体材料・力学研究会
    • Place of Presentation
      九州大学西新プラザ 福岡
    • Year and Date
      2015-07-28
    • Related Report
      2015 Annual Research Report
  • [Presentation] 「肝胆膵外科における医工連携の未来」2013

    • Author(s)
      山下 洋市、調 憲、米満 吉和、白木川 奈菜、井嶋 博之、前原 喜彦など
    • Organizer
      化学工学会第45回秋期大会
    • Place of Presentation
      岡山
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] 「肝胆膵外科における医工連携の未来」2013

    • Author(s)
      山下 洋市、調 憲、米満 吉和、白木川 奈菜、井嶋 博之、前原 喜彦など
    • Organizer
      INCHEM TOKYO 2013 産学官マッチングフォーラム
    • Place of Presentation
      東京
    • Related Report
      2013 Research-status Report
    • Invited

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Published: 2014-07-25   Modified: 2019-07-29  

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