| Project/Area Number |
25462096
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | Kyushu University |
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Principal Investigator |
Akahoshi Tomohiko 九州大学, 医学(系)研究科(研究院), 准教授 (20336019)
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| Co-Investigator(Kenkyū-buntansha) |
Hashizume Makoto 九州大学, 大学院医学研究院, 教授 (90198664)
Murata Masaharu 九州大学, 大学院医学研究院, 准教授 (30304744)
富川 盛雅 九州大学, 大学院医学研究院, 特任准教授 (60325454)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | 間葉系幹細胞 / 肝硬変 / 幹細胞治療 / 門脈圧亢進症 / 脂肪由来間葉系幹細胞 / 非代償肝硬変 / 肝増殖因子 |
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| Outline of Final Research Achievements |
Background:Recent studies show that adipose tissue-derived mesenchymal stem cells(ADSC) have potential clinical applications. Here, we investigated the effect of adipose tissue-derived mesenchymal stem cells infusion on a liver fibrosis rat model and elucidated the underlying mechanism.Result:bFGF treatment markedly improved the proliferation, differentiation, and hepatocyte growth factor expression ability of ADSC. Although ADSC infusion alone slightly ameliorated liver functions and suppressed fibrosis progression, bFGF-treatment significantly enhanced the therapeutic effect in association with elevated hepatocyte growth factor expression. Co-culture with ADSC led to induction of hepatic stellate cell apoptosis and enhanced hepatocyte proliferation. Mechanism investigation revealed that co-culture with adipose tissue-derived mesenchymal stem cells activated c-jun N-terminal kinase-p53 signaling in hepatic stellate cell and promoted apoptosis.
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