| Project/Area Number |
25462097
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | Nagasaki University |
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Principal Investigator |
YAMANOUCHI Kosho 長崎大学, 医歯薬学総合研究科(医学系), 講師 (10448508)
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| Co-Investigator(Kenkyū-buntansha) |
EGUCHI Susumu 長崎大学, 医歯薬学総合研究科(医学系), 教授 (80404218)
SAKAI Yusuke 長崎大学, 医歯薬学総合研究科(医学系), 助教 (10608904)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 再生医療 / 脂肪組織由来多能性幹細胞 / 肝放射線照射 / 増殖刺激 / 脂肪由来幹細胞 |
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| Outline of Final Research Achievements |
The selective proliferation of transplanted hepatocytes with a growth stimulus, such as portal vein branch ligation (PVBL), concomitant with hepatic irradiation (HIR), which can suppress proliferation of host hepatocytes, has been reported. We have conducted experiments that focused on less invasive and clinically applicable techniques and progenitor cells. First, DPP4-F344 rats underwent partial HIR and PVBL of one lobe, followed by intrasplenic hepatocyte transplantation at 1 × 10(7). Second, after partial HIR and PVBL, two types of progenitor cells were transplanted (small hepatocytes (SHs) or adipose-derived mesenchymal stem cells (ADSMC)).RESULTS:16 wk after transplantation, the donor cells constituted > 70% of the hepatocytes of the irradiated lobe, showing Cx32, PCK-1, and glycogen storage. The SHs grew more quickly than the hepatocytes. Although the ADSMCs were engrafted after 8 wk, their proliferation was not observed.
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