| Project/Area Number |
25462108
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | Shinshu University |
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Principal Investigator |
KOBAYASHI Akira 信州大学, 学術研究院医学系, 准教授 (90334903)
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| Co-Investigator(Kenkyū-buntansha) |
MOTOYAMA Hiroaki 信州大学, 学術研究院医学系(医学部附属病院), 助教 (20569587)
MIYAGAWA Shin-ichi 信州大学, 学術研究院医学系, 教授 (80229806)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2014: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2013: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
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| Keywords | 分化転換 / インスリン産生細胞 / 転写因子 / 再生医療 / 糖尿病 / β細胞 / 膵臓外科 |
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| Outline of Final Research Achievements |
The transcription factor (TFs)-mediated transdifferentiation is novel process to convert one adult cell type into an alternate cell type with a different function. This study focuses on the inducibility of TFs-mediated transdifferentiation using portal branch ligation-stimulated hepatic cells, which is bipotent hepatic progenitor cell clones, by culturing the cells in a serum-free differentiation medium containing specific compounds (SFDM) and/or using a three-dimensional (3D) cellular aggregation technique. We revealed that both treatment with SFDM and the use of a 3D culture acted interdependently to promote TFs-mediated transdifferentiation. This study provides an important strategy for the cellular transdifferentiation of adult somatic cells using defined factors.
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