New modality for cell-replacement therapy using adult liver-derived progenitor cells for post-pancreatectomy diabetic patients.

Research Project

Project/Area Number	25462108
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Research Field	Digestive surgery
Research Institution	Shinshu University
Principal Investigator	KOBAYASHI Akira 信州大学, 学術研究院医学系, 准教授 (90334903)
Co-Investigator(Kenkyū-buntansha)	MOTOYAMA Hiroaki 信州大学, 学術研究院医学系(医学部附属病院), 助教 (20569587) MIYAGAWA Shin-ichi 信州大学, 学術研究院医学系, 教授 (80229806)
Project Period (FY)	2013-04-01 – 2016-03-31
Project Status	Completed (Fiscal Year 2015)
Budget Amount *help	¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000) Fiscal Year 2015: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000) Fiscal Year 2014: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000) Fiscal Year 2013: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Keywords	分化転換 / インスリン産生細胞 / 転写因子 / 再生医療 / 糖尿病 / β細胞 / 膵臓外科
Outline of Final Research Achievements	The transcription factor (TFs)-mediated transdifferentiation is novel process to convert one adult cell type into an alternate cell type with a different function. This study focuses on the inducibility of TFs-mediated transdifferentiation using portal branch ligation-stimulated hepatic cells, which is bipotent hepatic progenitor cell clones, by culturing the cells in a serum-free differentiation medium containing specific compounds (SFDM) and/or using a three-dimensional (3D) cellular aggregation technique. We revealed that both treatment with SFDM and the use of a 3D culture acted interdependently to promote TFs-mediated transdifferentiation. This study provides an important strategy for the cellular transdifferentiation of adult somatic cells using defined factors.