| Project/Area Number |
25462112
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | Shimane University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
URANO Takeshi 島根大学, 医学部, 教授 (70293701)
NISHI Takeshi 島根大学, 医学部, 特別協力研究員 (80538893)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
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| Keywords | 胆道発がん / 胆汁耐性ヘリコバクター属菌 / ビリス菌 / ヘパティクス菌 / モノクローナル抗体 / 胆道発癌 |
|---|
| Outline of Final Research Achievements |
(1) We developed four or two monoclonal antibodies against Gram-negative enterohepatic Helicobacters, Helicobacter bilis or Helicobacter hepaticus, respectively. The Helicobacter hepaticus genome has been sequenced. For the epitope analysis the Helicobacter bilis genome was sequenced.
(2) We developed in vitro isolation and culture method of hamster bile duct epithelial cells. We performed a comprehensive analysis of upregulated or downregulated gene sets in these cells infected with Helicobacter bilis or Helicobacter hepaticus using DNA microarray.
(3) We developed two Helicobacter bilis or Helicobacter hepaticus infection models against hamster (5-week-old, female) and mouse (6-week-old, female).
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