Sensitivity of chemotherapy in pancreatic cancer cells
Project/Area Number |
25462128
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Digestive surgery
|
Research Institution | Fukuoka University |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
KUNIYASU Hiroki 奈良県立医科大学, 医学部, 教授 (00253055)
|
Project Period (FY) |
2013-04-01 – 2016-03-31
|
Project Status |
Completed (Fiscal Year 2015)
|
Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
Keywords | Gemcitabine耐性膵癌 / 3次元 / Gemcitabine耐性膵臓癌 |
Outline of Final Research Achievements |
We established GEM-resistant cells (MIA-GEMR) from MIA PaCa-2 cells and analyzed signaling pathway associated with the KRAS mutation MIA-GEMR cells showed the epithelial-mesenchymal transition phenotype and invasiveness, which were similarly induced by the KRAS mutation in colorectal cancer cells. The genes differentially expressed between MIA-GEMR and MIA PaCa-2 cells were compared with those observed between the human colorectal cancer HCT116 cells and HKe3 cells. Notably, the common genes associated with mutant KRAS regulation overlapped with stem cell-specific genes and TGF-β1 target genes, suggesting the crucial role of mutant KRAS in determining the morphology and microenvironment. Furthermore, the phosphodiesterase inhibitor resveratrol suppressed the growth of MIA-GEMR. These results suggest that TGF-β1 signaling downstream of oncogenic KRAS is involved in the onset of GEM resistance in MIA PaCa-2 cells.
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Report
(3 results)
Research Products
(7 results)
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[Journal Article] Pro chemotherapeutic effects of antibody against extracellular domain of claudin-4 in bladder cancer.2015
Author(s)
Kuwada M, Chihara Y, Luo Y, Li X, Nishiguchi Y, Fujiwara R, Sasaki T, Fujii K, Ohmori H, Fujimoto K, Kondoh M, Kuniyasu H.
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Journal Title
Cancer Lett.
Volume: 369
Pages: 212-221
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] Expression of MAS1 in breast cancer.2015
Author(s)
Luo Y, Tanabe E, Kitayoshi M, Nishiguchi Y, Fujiwara R, Matsushima S, Sasaki T, Sasahira T, Chihara Y, Nakae D, Fujii K, Ohmori H, Kuniyasu H.
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Journal Title
Cancer Sci.
Volume: 106
Pages: 1240-1248
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] Alteration of the plasma free amino acids profiles in cancer patients is associated with dysregulated metabolism in skeletal muscle.2015
Author(s)
Luo,Y., Yoneda,J., Sasaki,T., Kawahara,I., Goto,K., Fujii,K., Ohmori,H., Kuniyasu,H.
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Journal Title
Arch Cancer Res.
Volume: 3
Pages: 1-5
Related Report
Peer Reviewed / Int'l Joint Research
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