Development of therapeutic method for heart failure through cellular phenotype control using susutained release of microRNA
| Project/Area Number |
25462136
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Cardiovascular surgery
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| Research Institution | Kyoto University |
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Principal Investigator |
Yamazaki Kazuhiro 京都大学, 医学(系)研究科(研究院), 助教 (50464227)
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| Co-Investigator(Kenkyū-buntansha) |
ONO KOH 京都大学, 大学院医学研究科, 准教授 (00359275)
SAKATA RYUZO 京都大学, 大学院医学研究科, 名誉教授 (20325781)
TABATA YASUHIKO 京都大学, 再生医科学研究所, 教授 (50211371)
丸井 晃 京都大学, 医学(系)研究科(研究院), その他 (60402856)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 心筋疾患外科学 / 心筋リモデリング / 心不全 / 血管新生 / 心筋モデリング / マイクロＲＮＡ |
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| Outline of Final Research Achievements |
We performed the evaluation for optimal mixture condition of gelatin hydrogel and miR-29b using COS-7 cells. We could found the condition to efficiently induce miR-29b complementary plasmid within COS-7, providing suitable condition of better microRNA induction using sustained-release vehicles.
We next evaluated the efficiency of transduction of miR-126-incorporated poly(lactic-co-glycolic acid)(PLGA) for mice hindlimb ischemia model. Treatment with miR-126-incorporated PLGA led to increased blood flow and activation of signaling pathway related to miR-126 in gene and protein expression level. We showed fair efficiency of transduction of microRNA into the target cells which provides a basic knowledge for sustained release of microRNA into the heart muscle tissue.
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Report
(3 results)
Research Products
(23 results)
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[Journal Article] Safety and efficacy of sustained release of basic fibroblast growth factor using gelatin hydrogel in patients with critical limb ischemia.2016
Author(s)
Kumagai M, Marui A, Tabata Y, Takeda T, Yamamoto M, Yonezawa A, Tanaka S, Yanagi S, Ito-Ihara T, Ikeda T, Murayama T, Teramukai S, Katsura T, Matsubara K, Kawakami K, Yokode M, Shimizu A, Sakata R.
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Journal Title
Heart Vessels.
Volume: 未定
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Efficient long-term survival of cell grafts after myocardial infarction with thick viable cardiac tissue entirely from pluripotent stem cells.2015
Author(s)
Matsuo T, Masumoto H, Tajima S, Ikuno T, Katayama S, Minakata K, Ikeda T, Yamamizu K, Tabata Y, Sakata R, Yamashita JK.
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Journal Title
Scientific Reports
Volume: 5:16842
Issue: 1
Pages: 1-14
DOI
NAID
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] MicroRNA-33 regulates sterol regulatory element-binding protein 1 expression in mice.2013
Author(s)
Horie T, Nishino T, Baba O, Kuwabara Y, Nakao T, Nishiga M, Usami S, Izuhara M, Sowa N, Yahagi N, Shimano H, Matsumura S, Inoue K, Marusawa H, Nakamura T, Hasegawa K, Kume N, Yokode M, Kita T, Kimura T, Ono K
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Journal Title
Nat Commun.
Volume: 4:2883
Issue: 1
Pages: 2883-2883
DOI
NAID
Related Report
Peer Reviewed / Open Access
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[Journal Article] Metastasis-associated protein, S100A4 mediates cardiac fibrosis potentially through the modulation of p53 in cardiac fibroblasts2013
Author(s)
Tamaki Y, Iwanaga Y, Niizuma S, Kawashima T, Kato T, Inuzuka Y, Horie T, Morooka H, Takase T, Akahashi Y, Kobuke K, Ono K, Shioi T, Sheikh SP, Ambartsumian N, Lukanidin E, Koshimizu TA, Miyazaki S, Kimura T
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Journal Title
J Mol Cell Cardiol
Volume: 57
Pages: 72-81
DOI
Related Report
Peer Reviewed
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