Efficient cardiogenic differentiation from ES cells by three-dimensional culture in collagen hydrogel

• Project/Area Number: 25462142
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Cardiovascular surgery
• Research Institution: Nara Medical University
• Principal Investigator: TANIGUCHI SHIGEKI 奈良県立医科大学, 医学部, 教授 (90183467)
• Co-Investigator(Kenkyū-buntansha): 内藤 洋 奈良県立医科大学, 医学部, 助教 (00316069)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: 再生医療 / 組織工学 / 細胞・組織 / 心筋細胞 / ES細胞

Outline of Final Research Achievements

We showed that it was possible to induce differentiation into cardiomyocytes from undifferentiated ES cells by three-dimensional culture in collagen hydrogel. Undifferentiated ES cells formed cell aggregates in collagen hydrogel, and cell number increased slowly. We also showed that collagen concentration and initial cell seeding density affected differentiation ratio into cardiomyocyte, and we decided a culture condition which was possible to induce differentiation into cardiomyocyte with high efficiency. The addition of ascorbic acid increased the differentiation efficiency into cardiomyocyte. In three-dimensional culture the differentiation efficiency did not decrease in long term culture.