Improvement of damaged lung by type II alveolar epithelial cells with multipotency - for the purpose of clinical application of lung transplantation -
| Project/Area Number |
25462172
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Respiratory surgery
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| Research Institution | Chiba Cancer Center (Research Institute) (2016)
Chiba University (2013, 2015) |
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Principal Investigator |
Yoshida Shigetoshi 千葉県がんセンター(研究所), 呼吸器外科, 部長 (90334200)
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| Co-Investigator(Kenkyū-buntansha) |
坂入 祐一 千葉大学, 医学部附属病院, 助教 (30551949)
岩田 剛和 千葉県がんセンター(研究所), 呼吸器外科, 主任医長 (30586681)
吉野 一郎 千葉大学, 大学院医学研究院, 教授 (40281547)
鈴木 秀海 千葉大学, 医学部附属病院, 講師 (60422226)
鎌田 稔子 東京女子医科大学, 医学部, 助教 (60586692)
田川 哲三 九州大学, 医学研究院, 共同研究員 (90419557)
中島 崇裕 千葉大学, 大学院医学研究院, 講師 (20400913)
山本 高義 千葉県がんセンター(研究所), 呼吸器外科, 医長 (20648349)
尹 貴正 千葉大学, 医学部附属病院, 医員 (80710491)
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| Research Collaborator |
WADA HIRONOBU 千葉大学, 医学部附属病院, 助教 (90514604)
HATA ATSUSHI 千葉大学, 医学部附属病院, 医員 (30750806)
SHIINA YUKI 千葉大学, 医学部附属病院, 医員
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| Project Period (FY) |
2013-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 呼吸器外科学 / 移植 / 再生 / 肺移植 |
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| Outline of Final Research Achievements |
As a preliminary experiment, to stabilize the supply of type II alveolar epithelial cells (AEC-II) transferring in the injury lungs, we experimented the induction of differentiation from mouse induced pluripotent stem cells (iPS cells) to AEC-II. In addition, we established the mouse chronic obstructive bronchiolitis (BO) model after allogeneic lung transplantation, and the exhaustive gene analysis of the BO lung was performed. To image the kinetics of transferred AEC-II, the luciferase-transfected A549 cells were intravenously administered to mice, we confirmed the engraftment, distribution and proliferation of the cells in the lung.
We will be transferred ACE-II induced from iPS cells into mouse injury lung in next experiment, and the possibility of clinical application of cadaveric lung transplantation will be pursued.
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Report
(4 results)
Research Products
(25 results)
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[Journal Article] Role of complement activation in obliterative bronchiolitis post-lung transplantation.2013
Author(s)
Suzuki H, Lasbury ME, Fan L, Vittal R, Mickler EA, Benson HL, Shilling R, Wu Q, Weber DJ, Wagner SR, Lasaro M, Devore D, Wang Y, Sandusky GE, Lipking K, Pandya P, Reynolds J, Love R, Wozniak T, Gu H, Brown KM, Wilkes DS.
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Journal Title
J Immunol.
Volume: 191
Issue: 8
Pages: 4431-4439
DOI
Related Report
Peer Reviewed
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[Presentation] マウス肺全摘モデルを用いた代償性肺成長の解析2015
Author(s)
尹 貴正、鈴木秀海、畑敦, 稲毛輝長, 山本高義, 田中教久, 鎌田稔子, 森本淳一, 長門芳, 中島崇裕, 岩田剛和, 吉田成利, 吉野一郎
Organizer
第32回日本呼吸器外科学会総会
Place of Presentation
サンポートホール高松（香川県・高松市）
Year and Date
2015-05-15
Related Report
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[Presentation] Living donor lung transplantation for secondary pleuroparenchymal fi broelastosis after cytotoxic chemotherapy2015
Author(s)
Nakajima T, Yoshida S, Kinoshita T, Terada J, Tsushima K, Tanoue S, Tanaka E, Nagato K, Suzuki H, Iwata T, Date H, Yoshino I.
Organizer
The 11th Lung Transplantation Conference
Place of Presentation
サンポートホール高松（香川県・高松市）
Year and Date
2015-05-14
Related Report
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[Presentation] Donor lung derived myeloid and plasmacytoid dendritic cells differentially regulate T cell proliferation and cytokine production
Author(s)
Suzuki H, Yoshida S, Sata Y, Inage T, Yun T, Yamamoto T, Kamata T, Morimoto J, Sakairi Y, Nakajima T, Tagawa T, Iwata T, Mizobuchi T, Wilkes DS, Yoshino I.
Organizer
The 9th Lung Transplantation Conference
Place of Presentation
仙台
Related Report
