| Project/Area Number |
25462172
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Respiratory surgery
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| Research Institution | Chiba Cancer Center (Research Institute) (2016)
Chiba University (2013, 2015) |
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Principal Investigator |
Yoshida Shigetoshi 千葉県がんセンター(研究所), 呼吸器外科, 部長 (90334200)
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| Co-Investigator(Kenkyū-buntansha) |
坂入 祐一 千葉大学, 医学部附属病院, 助教 (30551949)
岩田 剛和 千葉県がんセンター(研究所), 呼吸器外科, 主任医長 (30586681)
吉野 一郎 千葉大学, 大学院医学研究院, 教授 (40281547)
鈴木 秀海 千葉大学, 医学部附属病院, 講師 (60422226)
鎌田 稔子 東京女子医科大学, 医学部, 助教 (60586692)
田川 哲三 九州大学, 医学研究院, 共同研究員 (90419557)
中島 崇裕 千葉大学, 大学院医学研究院, 講師 (20400913)
山本 高義 千葉県がんセンター(研究所), 呼吸器外科, 医長 (20648349)
尹 貴正 千葉大学, 医学部附属病院, 医員 (80710491)
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| Research Collaborator |
WADA HIRONOBU 千葉大学, 医学部附属病院, 助教 (90514604)
HATA ATSUSHI 千葉大学, 医学部附属病院, 医員 (30750806)
SHIINA YUKI 千葉大学, 医学部附属病院, 医員
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| Project Period (FY) |
2013-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 呼吸器外科学 / 移植 / 再生 / 肺移植 |
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| Outline of Final Research Achievements |
As a preliminary experiment, to stabilize the supply of type II alveolar epithelial cells (AEC-II) transferring in the injury lungs, we experimented the induction of differentiation from mouse induced pluripotent stem cells (iPS cells) to AEC-II. In addition, we established the mouse chronic obstructive bronchiolitis (BO) model after allogeneic lung transplantation, and the exhaustive gene analysis of the BO lung was performed. To image the kinetics of transferred AEC-II, the luciferase-transfected A549 cells were intravenously administered to mice, we confirmed the engraftment, distribution and proliferation of the cells in the lung.
We will be transferred ACE-II induced from iPS cells into mouse injury lung in next experiment, and the possibility of clinical application of cadaveric lung transplantation will be pursued.
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