The detection and analysis of the stromal cell included in the pulmonary venous blood of the resected lung cancer specimen.

• Project/Area Number: 25462184
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Respiratory surgery
• Research Institution: Keio University
• Principal Investigator: Kamiyama Ikuo 慶應義塾大学, 医学部, 講師 (70317147)
• Co-Investigator(Kenkyū-buntansha): KOHNO Mitsutomo 東海大学, 医学部, 准教授 (10276272) ; SUZUKI Shigeki 慶應義塾大学, 医学部, 助教 (80645672) ; WATANABE Masazumi 慶應義塾大学, 医学部, 教授 (90201227) ; 羽藤 泰 慶應義塾大学, 医学部, 助教 (10365281) ; 後藤 太一郎 慶應義塾大学, 医学部, 共同研究員 (80317148)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: 肺癌 / 転移性肺腫瘍 / ストローマ細胞

Outline of Final Research Achievements

The purpose of this study is to detect and analyze stroma cells included in the pulmonary venous blood of the resected lung from the lung cancer patients. We prepared mouse lung cancer model in order to establish to detect stromal cells circulating with cancer cells in the pulmonary venous blood. Incubated lung cancer cells, Lewis lung carcinoma (LLC), were suspended with Matrigel and injected into mouse lung parenchyma. Cancer cells formed tumor, then the tumor was increased in size chronologically. Pathological result indicated the engraftment of cancer cells into mouse lung parenchyma. Subsequently we tried to detect the cancer cells using the cell block technique in pulmonary venous blood. We made cell block from pulmonary venous blood, but it was difficult to obtain cancer cells because of smallness of circulating cancer cells. Further studies should be conducted to validate this method for screening circulating stromal cells.

Research Products

• [Journal Article] Comprehensive Immune Profiling of Lung Adenocarcinomas Reveals Four Immunosubtypes with Plasma Cell Subtype a Negative Indicator. (2016)
  • Author(s): Kurebayashi Y, Emoto K, Hayashi Y, Kamiyama I, Ohtsuka T, Asamura H, Sakamoto M.
  • Journal Title: Cancer Immunol Res. Volume: 4(3) Issue: 3 Pages: 234-237
  • DOI: 10.1158/2326-6066.cir-15-0214
  • Peer Reviewed
• [Journal Article] Prognostic significance of hypoxic PET using (18)F-FAZA and (62)Cu-ATSM in non-small-cell lung cancer. (2016)
  • Author(s): Kinoshita T, Fujii H, Hayashi Y, Kamiyama I, Ohtsuka T, Asamura H.
  • Journal Title: Lung Cancer Volume: 91 Pages: 56-66
  • DOI: 10.1016/j.lungcan.2015.11.020
  • Peer Reviewed
• [Journal Article] Impact of comorbidity index on morbidity and survival in non-small cell lung cancer. (2016)
  • Author(s): Otake S, Ohtsuka T, Asakura K, Kamiyama I, Kohno M.
  • Journal Title: Asian Cardiovasc Thorac Ann. Volume: 24(1) Issue: 1 Pages: 238-238
  • DOI: 10.1177/0218492315617834
  • Peer Reviewed
• [Journal Article] Alveolar macrophage phenotype expression in airway-instilled bone marrow cells in mice. (2015)
  • Author(s): Okui M, Goto T, Asakura K, Kamiyama I, Ohtsuka T.
  • Journal Title: Springerplus Volume: 4 Issue: 1 Pages: 770-770
  • DOI: 10.1186/s40064-015-1525-2
  • Peer Reviewed / Open Access
• [Journal Article] Survival predictors after resection of lung metastases of head or neck cancers. (2015)
  • Author(s): Yotsukura M, Kinoshita T, Kohno M, Asakura K, Kamiyama I, Emoto K, Hayashi Y, Ohtsuka T.
  • Journal Title: Thorac Cancer. Volume: 6(5) Issue: 5 Pages: 579-583
  • DOI: 10.1111/1759-7714.12225
  • Peer Reviewed / Open Access
• [Journal Article] Thirty-day outcomes after lobectomy or segmentectomy for lung cancer surgery. (2015)
  • Author(s): Ohtsuka T, Kamiyama I, Asakura K, Kohno M.
  • Journal Title: Asian Cardiovasc Thorac Ann. Volume: 23(7) Issue: 7 Pages: 828-831
  • DOI: 10.1177/0218492315589476
  • Peer Reviewed
• [Journal Article] Prognostic impact of preoperative tumor marker levels and lymphovascular invasion in pathological stage I adenocarcinoma and squamous cell carcinoma of the lung. (2015)
  • Author(s): Kinoshita T, Ohtsuka T, Yotsukura M, Asakura K, Goto T, Kamiyama I, Otake S, Tajima A, Emoto K, Hayashi Y, Kohno M.
  • Journal Title: J Thorac Oncol. Volume: 10(4) Issue: 4 Pages: 619-628
  • DOI: 10.1097/jto.0000000000000480
• [Presentation] Multimodality Treatment for Advanced Thymoma (2015)
  • Author(s): Ikuo Kamiyama, Takashi Ohtsuka, Takao Shigenobu, Yasoo Sugiura and Hisao Asamura
  • Organizer: 16TH WORLD CONFERENCE ON LUNG CANCER
  • Place of Presentation: デンバー（アメリカ合衆国）
  • Year and Date: 2015-09-06
  • Int'l Joint Research
• [Presentation] 肺癌手術におけるハーモニックスカルペル（フックタイプDH105）を用いた安全な肺門部露出法 (2015)
  • Author(s): 神山育男、青木耕平、澤藤誠、淺村尚生
  • Organizer: 第32回日本呼吸器外科学会総会
  • Place of Presentation: サンポートホール高松（香川県高松市）
  • Year and Date: 2015-05-14
• [Presentation] 進行非小細胞肺癌に対してgefitinib投与後にサルベージ肺切除を施行した2例の経過 (2013)
  • Author(s): 河野 光智, 大竹 宗太郎, 松田 信作, 四倉 正也, 重信 敬夫, 木下 智成, 加勢田 馨, 奥井 将之, 羽藤 泰, 神山 育男, 後藤 太一郎, 大塚 崇, 渡辺 真純, 堀之内 宏久
  • Organizer: 第54回日本肺癌学会学術集会
  • Place of Presentation: 東京
• [Presentation] 葉切除または区域切除による術後30日以内の合併症の比較検討 (2013)
  • Author(s): 大塚 崇, 羽藤 泰, 神山 育男, 後藤 太一郎, 河野 光智
  • Organizer: 第54回日本肺癌学会学術集会
  • Place of Presentation: 東京
• [Presentation] 肺尖部胸壁浸潤肺癌手術症例の検討 (2013)
  • Author(s): 神山 育男, 大竹 宗太郎, 松田 信作, 四倉 正也, 重信 敬夫, 加勢田 馨, 木下 智成, 奥井 将之, 羽藤 泰, 後藤 太一郎, 大塚 崇, 河野 光智
  • Organizer: 第54回日本肺癌学会学術集会
  • Place of Presentation: 東京
• [Presentation] 頭頸部原発悪性腫瘍の肺転移に対する肺切除例の検討 (2013)
  • Author(s): 四倉 正也, 木下 智成, 大竹 宗太郎, 松田 信作, 重信 敬夫, 加勢田 馨, 奥井 将之, 羽藤 泰, 神山 育男, 後藤 太一郎, 大塚 崇, 江本 桂, 林 雄一郎, 河野 光智
  • Organizer: 第54回日本肺癌学会学術集会
  • Place of Presentation: 東京
• [Presentation] 末梢発生肺扁平上皮癌における血管・胸膜浸潤の意義 (2013)
  • Author(s): 木下 智成, 加勢田 馨, 羽藤 泰, 神山 育男, 後藤 太一郎, 大塚 崇, 林 雄一郎, 田島 敦志, 河野 光智
  • Organizer: 第54回日本肺癌学会学術集会
  • Place of Presentation: 東京