| Project/Area Number |
25462185
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Respiratory surgery
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| Research Institution | Juntendo University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
SUZUKI Kenji 順天堂大学, 医学部, 教授 (10415523)
KOGO Yasushi 独立行政法人理化学研究所, オミックス基盤研究領域, 研究員 (20462682)
KAWAI Jun 独立行政法人理化学研究所, オミックス基盤研究領域, 研究員 (30391923)
Oh Shiaki 順天堂大学, 医学部, 准教授 (50306958)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 肺癌 / 腺癌 / 扁平上皮癌 / 鑑別マーカー / SPATS2 / ST6GALNAC1 / 転移性肺腫瘍 / CAGE / 国内共同研究 |
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| Outline of Final Research Achievements |
Precisely diagnosing squamous cell carcinoma (SCC) and adenocarcinoma (AD) is important to select the most effective treatment regimen for lung cancer, but this has been challenging in cases with poorly differentiated SCC (PDSCC) and AD without a lepidic growth component (non-lepidic AD). We approached this problem with a genome-wide technology to monitor promoter activities, Cap Analysis Gene Expression (CAGE). Based on the genome-wide profiles of 97 frozen tissues from surgically resected lung cancers, we identified two novel markers, SPATS2 for SCC and ST6GALNAC1 for AD. The expression of known molecular markers used in immunohistochemical analysis (IHC) for SCC (CK5, CK6, p40 and desmoglein-3) and AD (TTF-1 and napsin A) were different between SCC and AD. We confirmed their performance with an IHC and also confirmed the findings in another group of 74 patients.
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