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Sox2 is a novel target gene for the treatment of lung squamous cell carcinoma.

Research Project

Project/Area Number 25462188
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Respiratory surgery
Research InstitutionKawasaki Medical School

Principal Investigator

Fukazawa Takuya  川崎医科大学, 医学部, 講師 (20379845)

Co-Investigator(Kenkyū-buntansha) NAOMOTO YOSHIO  川崎医科大学, 医学部, 教授 (00237190)
YAMATSUJI TOMOKI  川崎医科大学, 医学部, 准教授 (40379730)
TAKAOKA MUNENORI  川崎医科大学, 医学部, 講師 (50548568)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywords肺扁平上皮癌 / Sox2遺伝子
Outline of Final Research Achievements

Sox2 is a pluripotency controller that was recently identified as a novel oncogene, recurrently activated in lung squamous cell carcinoma (SCC). Inhibition of Sox2 by siRNA suppresses cell viability and colony formation of Sox2 expressing EBC2 and LK2 lung SCC cells. Moreover, Sox2 siRNA inhibits lung SCC growth in vivo in a xenograft mouse model derived from these cells. Cell cycle analysis showed that Sox2 silencing significantly increased the G1 population in the cells. We used biological studies to demonstrate that CDKN1A was suppressed by Sox2 in lung SCC cells. G1 cell cycle arrest induced by Sox2 siRNA was rescued by CDKN1A siRNA. These results indicate that targeting Sox2 is an effective strategy for the treatment of Sox2-expressing lung SCC and the tumorigenic effect of Sox2 in lung SCC cells is mediated in part by suppression of CDKN1A.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • Research Products

    (2 results)

All 2016 2015

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (1 results)

  • [Journal Article] SOX2 suppresses CDKN1A to sustain growth of lung squamous cell carcinoma.2016

    • Author(s)
      Fukazawa T, Guo M, Ishida N, Yamatsuji T, Takaoka M, Yokota E, Haisa M, Miyake N, Ikeda T, Okui T, Takigawa N, Maeda Y, Naomoto Y.
    • Journal Title

      Scientific Reports

      Volume: 6 Pages: 20113-20113

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Presentation] 人工転写因子を用いた肺扁平上皮癌に対する新規標的療法の開発2015

    • Author(s)
      横田 悦子1、深澤 拓也1、山辻 知樹1、高岡 宗徳1、羽井佐 実1、三 宅 規子2、池田 智子2、石田 尚正1、吉田 将和1、瀧川 奈義夫3、世 良 貴史4、猶本 良夫1 (1 川崎医科大学・総合外科、2 川崎病院研究セ ンター、3 川崎医科大学・総合内科 4、4 岡山大学・生命工学・生体機 能分子設計)
    • Organizer
      第74回日本癌学会学術総会
    • Place of Presentation
      名古屋国際会議場(愛知県名古屋市)
    • Year and Date
      2015-10-10
    • Related Report
      2015 Annual Research Report

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Published: 2014-07-25   Modified: 2019-07-29  

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