| Project/Area Number |
25462191
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Respiratory surgery
|
|---|
| Research Institution | University of Occupational and Environmental Health, Japan |
|---|
Principal Investigator |
SO Tomoko 産業医科大学, 医学部, 講師 (00341529)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
TANAKA Fumihiro 産業医科大学, 医学部, 教授 (10283673)
URAMOTO Hidetaka 産業医科大学, 医学部, 准教授 (90389445)
CHIKAISHI Yasuhiro 産業医科大学, 医学部, 助教 (25861251)
YONEDA Kazue 産業医科大学, 医学部, 助教 (80724806)
近石 泰弘 産業医科大学, 医学部, 助教 (70609221)
|
|---|
| Research Collaborator |
KATAYORI Satoshi JSRライフサイエンス株式会社, 研究開発部
|
|---|
| Project Period (FY) |
2013-04-01 – 2016-03-31
|
| Project Status |
Completed (Fiscal Year 2015)
|
| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
|
|---|
| Keywords | 肺癌 / 循環腫瘍細胞 / EpCAM / 細胞分離 / 細胞分離用磁性粒子 / 上皮間葉系移行(EMT) / 悪性胸膜中皮腫 |
|---|
| Outline of Final Research Achievements |
The “CellSearch” is widely used to detect circulating tumor cells (CTCs), but provided only modest sensitivity in capturing CTCs in lung cancer patients. In the current study, we employed a variety of CTC-detection systems including a novel microfluidic device (“CTC-chip”) in which any antibody to capture CTCs is easily conjugated. In experimental studies using tumor cell lines, the “CTC-chip” provided a similar efficiency to the “CellSearch” in capturing PC-9 cells with EpCAM expression. When MESO-4 cells without EpCAM expression were spiked in the blood, approximately 80% of tumor cells were captured with the “CTC-chip”, whereas few tumor cells were captured with the “CellSearch”.
|
