The antitumor effect of PI3K/mTOR inhibitor against malignant pleural mesothelioma
| Project/Area Number |
25462200
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Respiratory surgery
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| Research Institution | Hyogo Medical University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
近藤 展行 兵庫医科大学, 医学部, 講師 (50402889)
松本 成司 兵庫医科大学, 医学部, 講師 (60412011)
多久和 輝尚 兵庫医科大学, 医学部, 講師 (00412049)
橋本 昌樹 兵庫医科大学, 医学部, 助教 (40461074)
黒田 鮎美 兵庫医科大学, 医学部, 助教 (90642570)
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| Project Period (FY) |
2013-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 中皮腫 / 悪性胸膜中皮腫 |
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| Outline of Final Research Achievements |
We could confirm the antitumor effect of mTOR inhibitor against malignant pleural mesothelioma cell lines in vivo and mouse subcutaneous model. We focused on a PI3K/mTOR inhibitor that inhibits both mTOR and PI3K, which is upstream of mTOR, as a measure for possible resistance development. In H2052, which is most sensitive to PI3K/mTOR inhibitors, expression of gene (F) was decreased compared with other cell lines. When gene (F) was inactivated in the wild-type cell line, enhancement of mTOR phosphorylation was confirmed. In immunostaining of surgical specimens, multivariate analysis suggested that p-mTOR could be a therapeutic target. In gene (F)-deficient mesothelioma cell line, subcutaneous tumor growth was significantly reduced by treatment with a PI3K/mTOR inhibitor compared to untreated group.
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Report
(4 results)
Research Products
(2 results)
