Analysis of magnesium-induced vasodilation and dysfunction in cerebral penetrating arterioles after subarachnoid hemorrhage

• Project/Area Number: 25462210
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Neurosurgery
• Research Institution: Shinshu University
• Principal Investigator: Murata Takahiro 信州大学, 学術研究院医学系, 助教 (80533322)
• Co-Investigator(Kenkyū-buntansha): HORIUCHI Tetsuyoshi 信州大学, 学術研究院医学系, 准教授 (40303466)
• Research Collaborator: Dietrich Hans H.
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: マグネシウム / 脳細動脈 / くも膜下出血 / 脳血管攣縮 / 細動脈血管内皮

Outline of Final Research Achievements

We investigated in cerebral penetrating arterioles the signaling mechanisms and dose-dependency of extracellular magnesium-induced vasodilation and also its vasodilatory effects in vessels preconstricted with agonists associated with delayed cerebral vasospasm following subarachnoid hemorrhage (SAH).
Male rat penetrating arterioles were cannulated. Their internal diameters were monitored.
Magnesium induced a concentration- and smooth muscle cell-dependent dilation in cerebral penetrating arterioles. Calcium-sensitive potassium channels of smooth muscle cells may play a key role in magnesium-induced vasodilation. Magnesium also dilated endothelium-impaired vessels as well as vessels preconstricted with spasmogenic agonists. These results provide a fundamental background for the clinical use of magnesium, especially in treatment against delayed cerebral ischemia or vasospasm following SAH.

Research Products

• [Journal Article] Mechanisms of magnesium-induced vasodilation in cerebral penetrating arterioles. (2016)
  • Author(s): Murata T, Dietrich HH, Horiuchi T, Hongo K, Dacey RG Jr.
  • Journal Title: Neuroscience Research Volume: Epub ahead of print Pages: 57-62
  • DOI: 10.1016/j.neures.2015.12.005
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant