Development of regenerative medicine by oligodendrocyte and schwann precursor cells

• Project/Area Number: 25462215
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Neurosurgery
• Research Institution: Osaka University
• Principal Investigator: Ohnishi Yuichiro 大阪大学, 医学(系)研究科(研究院), 助教 (00533811)
• Co-Investigator(Kenkyū-buntansha): Yoshimine Toshiki (Shinzawa Koei) 大阪大学, 医学系研究科, 教授 (00201046) ; Kishima Haruhiko 大阪大学, 医学系研究科, 講師 (10332743) ; Iwatsuki Koichi 大阪大学, 医学系研究科, 准教授 (80346204) ; 新沢 康英 大阪大学, 医学(系)研究科(研究院), 助教 (70403186)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 嗅粘膜 / 神経幹細胞 / オリゴデンドロサイト前駆細胞 / GABAニューロン / オルファクトリースフィア / オリゴデンドロサイト / シュワン細胞 / ニューロン

Outline of Final Research Achievements

The olfactory epithelial layer contains multipotent horizontal base cells(HBCs) that differentiate into olfactory sensory neurons. We have identified that HBCs express oligodendrocyte precursor cell (OPC) and astrocyte markers.

Research Products

• [Journal Article] Isolation of human adult olfactory sphere cells as a cell source of neural progenitors. (2015)
  • Author(s): Ohnishi Y1, Iwatsuki K2, Ishihara M2, Shikina T3, Shinzawa K4, Moriwaki T2, Ninomiya K2, Ohkawa T2, Umegaki M2, Kishima H2, Yoshimine T2.
  • Journal Title: Stem Cell Research Volume: 15(1) Issue: 1 Pages: 23-29
  • DOI: 10.1016/j.scr.2015.04.006
  • Peer Reviewed / Open Access
• [Journal Article] Olfactory sphere cells are a cell source for γ-aminobutyric acid-producing neurons. (2015)
  • Author(s): Ohnishi YI, Maruo T, Shinzawa K, Iwatsuki K, Moriwaki T, Oshino S, Kishima H, YoshimineT
  • Journal Title: J Neurosci Res. 2 Volume: 93 Issue: 8 Pages: 1293-1304
  • DOI: 10.1002/jnr.23585
  • Peer Reviewed
• [Journal Article] A case of intrapelvic sciatic nerve schwannoma presenting as piriformis syndrome. (2014)
  • Author(s): 1. Ninomiya K, Iwatsuki K, Ohnishi Y, Moriwaki T, Yoshimine T.
  • Journal Title: Journal of Neuroscinece and Behavioural Health. Volume: 6 Pages: 1-4
  • Peer Reviewed
• [Journal Article] Adult olfactory sphere cells are a source of oligodendrocyte and Schwann cell progenitors. (2013)
  • Author(s): Ohnishi Y, Iwatsuki K et al.
  • Journal Title: Stem Cell Research Volume: 11 Issue: 3 Pages: 1178-1190
  • DOI: 10.1016/j.scr.2013.08.005
  • Peer Reviewed
• [Journal Article] Intradural extramedullary spinal ependymoma: a case report of malignant transformation occurring. (2013)
  • Author(s): 7. Moriwaki T, Iwatsuki K, Ohnishi Y, Umegaki M, Ishihara M, Yoshimine T.
  • Journal Title: Asian Spine Journal Volume: 7 Issue: 2 Pages: 139-42
  • DOI: 10.4184/asj.2013.7.2.139
  • Peer Reviewed
• [Journal Article] Transplantation of Olfactory Mucosa as a Scaffold for Axonal Regeneration Following Spinal Cord Contusion in Rats. (2013)
  • Author(s): 5. Iwatsuki K, Yoshimine T, Sankai Y, Umegaki M, Ohnishi Y, Ishihara M, Moriwaki T and Oda N.
  • Journal Title: Neuroscience & Medicine. Volume: 4 Pages: 112-116
• [Journal Article] Involuntary muscle spasm expressed as motor evoked potential after olfactory mucosa autograft in patients with chronic spinal cord injury and complete paraplegia. (2013)
  • Author(s): 4. Iwatsuki K, Yoshimine T, Sankai Y, Tajima F, Umegaki M, Ohnishi Y, Ishihara M, Ninomiya K and Moriwaki T.
  • Journal Title: Journal of Biomedical Science and Engineering. Volume: 6 Pages: 908-916
• [Presentation] 慢性期脊髄損傷に対する自己嗅粘膜移植術 (2013)
  • Author(s): 大西諭一郎
  • Organizer: 第67回近畿脊髄外科研究会
  • Place of Presentation: 大阪
  • Invited
• [Presentation] オルファクトリースフィア細胞による中枢・末梢神経損傷治療の検討 (2013)
  • Author(s): 大西諭一郎
  • Organizer: 第28回日本脊髄外科学会
  • Place of Presentation: 名古屋
• [Presentation] THE STEM CELL PROPETIES OF ADULT OLFACTORY HORIZONTAL BASAL CELLS (2013)
  • Author(s): Yu-ichiro Ohnishi
  • Organizer: Glia meeting 2013
  • Place of Presentation: Berlin
• [Presentation] 脊髄円錐部グリオーマの一例 (2013)
  • Author(s): 大西諭一郎
  • Organizer: 第66回日本脳神経外科学会近畿支部学術集会
  • Place of Presentation: 大阪
• [Presentation] 当院での脊髄星細胞腫の治療 (2013)
  • Author(s): 大西諭一郎
  • Organizer: 第72回日本脳神経外科学会
  • Place of Presentation: 横浜
• [Presentation] オルファクトリースフィア細胞による中枢・末梢神経損傷治療の検討 (2013)
  • Author(s): 大西諭一郎
  • Organizer: 第14回日本分子脳神経外科学会
  • Place of Presentation: 横浜
• [Book] 脊椎脊髄ジャーナル (2013)
  • Author(s): 大西諭一郎
  • Total Pages: 5
  • Publisher: 三輪書店